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胚胎干细胞培养技术大全

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2646

  • MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
  • Routine Culturing of ES Cells
  • ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
  • MITOMYCIN C TREATMENT OF PMEFs
  • GAMMA IRRADIATION OF PMEF'S
  • PMEF FEEDER LAYER CONCENTRATION
  • ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
  • KARYOTYPING ES CELLS
  • VASECTOMY OF MALE MICE
  • Normaski Optics
  • MEDIA FOR EMBRYO CULTURE AND MANIPULATION
  • Suppliers
  • PREPARATION OF MICROINJECTION PIPETTES
  • EMBRYO MANIPULATION PIPETTES (FLUSHERS ETC)
  • COLLECTION OF MORULAE and Earlier
  • Aggregation of ES cells and eight cell stage embryos
  • BLASTOCYST TRANSFER
  • Sending sera to testing for mouse pathogens.
  • SUPEROVULATION OF MICE
  • FLUSHING OUT OF BLASTOCYSTS
  • Alizarin Red S (bone) and alcian blue (cartilagestaining of cleared skeletons.
  • Routine Splitting and freezing of cells
  • SOFT AGAR ASSAY FOR COLONY FORMATION
  • VIRUS INFECTION OF FIBROBLASTS
  • DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
  • LIPOFECTAMINE FOR TRANSFORMATION OF EUKARYOTIC CELLS
  • ELECTROPORATION OF COS-7 CELLS
  • Growth and transfection of PC12 cells.
  • CELL CULTURE FROM WHOLE MICE EMBRYO (DAY 10 - 11 PC)
  • PCR GENOTYPING FROM the EMBRYO YOKE SACK
  • b-gal staining of eukaryotic cells in vitro
  • Comments on db-cAMP and forskolin
  • Comments on Ro 31.8220
  • IMMUNOFLUORESENCE ON CHAMBER SLIDES
  • PHOTOMICROGRAPHY
  • Reagents:
  • 干细胞相关仪器试剂耗材

    • 细胞培养皿
    • 细胞培养系统
    • 细胞计数
    • 干细胞/祖细胞
    • 干细胞培养基
    • 干细胞分化 干细胞扩增
    • 干细胞鉴定
    • 干细胞培养质控
    • 干细胞培养添加物
    • 干细胞培养附件
    • 细胞因子、趋化因子和生长因子
    • 细胞培养添加物
    • siRNA库

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