胚胎干细胞培养技术大全
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- MEDIA AND SOLUTIONS REQUIRED FOR ROUTINE ES CELL CULTURE
- Routine Culturing of ES Cells
- ISOLATION OF PRIMARY MOUSE EMBRYO FIBROBLASTS
- MITOMYCIN C TREATMENT OF PMEFs
- GAMMA IRRADIATION OF PMEF'S
- PMEF FEEDER LAYER CONCENTRATION
- ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
- KARYOTYPING ES CELLS
- VASECTOMY OF MALE MICE
- Normaski Optics
- MEDIA FOR EMBRYO CULTURE AND MANIPULATION
- Suppliers
- PREPARATION OF MICROINJECTION PIPETTES
- EMBRYO MANIPULATION PIPETTES (FLUSHERS ETC)
- COLLECTION OF MORULAE and Earlier
- Aggregation of ES cells and eight cell stage embryos
- BLASTOCYST TRANSFER
- Sending sera to testing for mouse pathogens.
- SUPEROVULATION OF MICE
- FLUSHING OUT OF BLASTOCYSTS
- Alizarin Red S (bone) and alcian blue (cartilagestaining of cleared skeletons.
- Routine Splitting and freezing of cells
- SOFT AGAR ASSAY FOR COLONY FORMATION
- VIRUS INFECTION OF FIBROBLASTS
- DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOSPHATE
- LIPOFECTAMINE FOR TRANSFORMATION OF EUKARYOTIC CELLS
- ELECTROPORATION OF COS-7 CELLS
- Growth and transfection of PC12 cells.
- CELL CULTURE FROM WHOLE MICE EMBRYO (DAY 10 - 11 PC)
- PCR GENOTYPING FROM the EMBRYO YOKE SACK
- b-gal staining of eukaryotic cells in vitro
- Comments on db-cAMP and forskolin
- Comments on Ro 31.8220
- IMMUNOFLUORESENCE ON CHAMBER SLIDES
- PHOTOMICROGRAPHY
- Reagents:
- 细胞培养皿
- 细胞培养系统
- 细胞计数
- 干细胞/祖细胞
- 干细胞培养基
- 干细胞分化 干细胞扩增
- 干细胞鉴定
- 干细胞培养质控
- 干细胞培养添加物
- 干细胞培养附件
- 细胞因子、趋化因子和生长因子
- 细胞培养添加物
- siRNA库
干细胞相关仪器试剂耗材
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