聚丙烯酰胺凝胶电泳凝胶的配制
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配制 Tris - 甘氨酸 SDS-PAGE 聚丙烯酰胺凝胶电泳分离胶所用溶液
|
溶液成分 |
不同体积（ ml ）凝胶液中各成分所需体积（ ml ） |
|||||||
|
5 |
10 |
15 |
20 |
25 |
30 |
40 |
50 |
|
|
6% SDS-PAGE |
|
|
|
|
|
|
|
|
|
水 |
2.6 |
5.3 |
7.9 |
10.6 |
13.2 |
15.9 |
21.2 |
26.5 |
|
30% 丙烯酰胺溶液 |
1 |
2 |
3 |
4 |
5 |
6 |
8 |
10 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% 过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.004 |
0.008 |
0.012 |
0.016 |
0.02 |
0.024 |
0.032 |
0.04 |
|
|
|
|
|
|
|
|
|
|
|
8% SDS-PAGE |
|
|
|
|
|
|
|
|
|
水 |
2.3 |
4.6 |
6.9 |
9.3 |
11.5 |
13.9 |
18.5 |
23.2 |
|
30% 丙烯酰胺溶液 |
1.3 |
2.7 |
4 |
5.3 |
6.7 |
8 |
10.7 |
13.3 |
|
1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% 过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.003 |
0.006 |
0.009 |
0.012 |
0.015 |
0.018 |
0.024 |
0.03 |
|
|
|
|
|
|
|
|
|
|
|
10% SDS -PAGE |
|
|
|
|
|
|
|
|
|
水 |
1.9 |
4 |
5.9 |
7.9 |
9.9 |
11.9 |
15.9 |
19.8 |
|
30% 丙烯酰胺溶液 |
1.7 |
3.3 |
5 |
6.7 |
8.3 |
10 |
13.3 |
16.7 |
|
1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% 过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
|
|
|
|
|
|
|
|
|
|
|
12% SDS-PAGE |
|
|
|
|
|
|
|
|
|
水 |
1.6 |
3.3 |
4.9 |
6.6 |
8.2 |
9.9 |
13.2 |
16.5 |
|
30% 丙烯酰胺溶液 |
2 |
4 |
6 |
8 |
10 |
12 |
16 |
20 |
|
1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% 过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
|
|
|
|
|
|
|
|
|
|
|
15% SDS-PAGE |
|
|
|
|
|
|
|
|
|
水 |
1.1 |
2.3 |
3.4 |
4.6 |
5.7 |
6.9 |
9.2 |
11.5 |
|
30% 丙烯酰胺溶液 |
2.5 |
5 |
7.5 |
10 |
12.5 |
15 |
20 |
25 |
|
1.5 mol/L Tris (pH8.8) |
1.3 |
2.5 |
3.8 |
5 |
6.3 |
7.5 |
10 |
12.5 |
|
10% SDS |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
10% 过硫酸氨 |
0.05 |
0.1 |
0.15 |
0.2 |
0.25 |
0.3 |
0.4 |
0.5 |
|
TEMED |
0.002 |
0.004 |
0.006 |
0.008 |
0.01 |
0.012 |
0.016 |
0.02 |
