Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers

Zone electrophoresis is the basic mode of capillary electrophoresis, and it allows separation of ions having different charge-to-size ratios. Carbohydrates having ionic groups can also be the objects of separation by this mode. A typical example is the separation of carboxylated/sulfated oligosaccharides from glycosaminoglycans, as described in Chapter 15 . Most carbohydrates, however, have no electric charge under normal conditions. Therefore, introduction of ionic tags plays an important role in zone electrophoresis of such carbohydrates.