Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
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Zone electrophoresis is the basic mode of capillary electrophoresis, and it allows separation of ions having different charge-to-size
ratios. Carbohydrates having ionic groups can also be the objects of separation by this mode. A typical example is the separation
of carboxylated/sulfated oligosaccharides from glycosaminoglycans, as described in Chapter 15
. Most carbohydrates, however, have no electric charge under normal conditions. Therefore, introduction of ionic tags plays
an important role in zone electrophoresis of such carbohydrates.