丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Harvesting GPI-Anchored Proteins From CHO Cells

互联网

421
Glycosyl-phosphatidylinositol (GPI)-anchored proteins bound to the outer surface of cell membranes (1 ,2 ) can be selectively released in a soluble form by the action of a highly specific bacterial enzyme, phosphatidylinositol-phospholipase C (PI-PLC) (3 ,4 ). This chapter describes a controlled release process (5 7 ) that increases both the concentration and purity of harvested GPI-anchored proteins by separating the harvesting of the protein from cell growth and protein expression. This approach has obtained product concentrations of up to 500 μg/mL at purities of 35% total protein (5 ,7 ).
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序