抗体标记方法

• Biotinylation of Antibody ( Contributed by Nanci Donacki )
  
• Antibody labeling (House Ear Institute)
  Labeling protocols using affinity markers.
  
• Coupling Colloidal Gold to Protein (Walter Steffen)
  • Gold labelling of rabbit IgG
  • Preparation of enzyme gold complex
  • Coupling of Nanogold to IgG
    
• Production of Colloidal Gold (Walter Steffen)
  
• Protein (Antibody) Fluorescent Labeling (Mario Roederer)
  Detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
  • Fluorescein (FITC)
  • Phycoerythrin (PE)
  • Cy5PE
  • Cy7PE
  • Texas Red (TR)
  • Allophycocyanin (APC)
  • Cy5
  • Cy7APC
  • Cascade Blue
  • Biotin

In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins.

• FITC
• PE
• TR-BSA
• APC
• Cascade Blue