高手请进：评价设计的引物？急！！！

预扩增包含8344位点的序列：
8101 agatgcaatt cccggacgtc taaaccaaac cactttcacc gctacacgac cgggggtata
8161 ctacggtcaa tgctctgaaa tctgtggagc aaaccacagt ttcatgccca tcgtcctaga
8221 attaattccc ctaaaaatct ttgaaatagg gcccgtattt accctatagc accccctcta
8281 ccccctctag agcccactgt aaagctaact tagcattaac cttttaagtt aaagattaag
8341 agaaccaaca cctctttaca gtgaaatgcc ccaactaaat actaccgtat ggcccaccat
8401 aattaccccc atactcctta cactattcct catcacccaa ctaaaaatat taaacacaaa
8461 ctaccaccta cctccctcac caaagcccat aaaaataaaa aattataaca aaccctgaga
8521 accaaaatga acgaaaatct gttcgcttca ttcattgccc ccacaatcct aggcctaccc
在网上premier3.0软件查询的引物如下：
No mispriming library specified
Using 1-based sequence positions
WARNING: Unrecognized base in input sequence

OLIGO start len tm gc% any 3' seq
LEFT PRIMER 26 20 59.87 45.00 3.00 2.00 aaaccaaaccactttcaccg
RIGHT PRIMER 422 20 59.93 45.00 2.00 0.00 ttttatgggctttggtgagg
SEQUENCE SIZE: 512
INCLUDED REGION SIZE: 512
PRODUCT SIZE: 397, PAIR ANY COMPL: 6.00, PAIR 3' COMPL: 2.00
1 8101agatgcaattcccggacgtctaaaccaaaccactttcaccgctacacgaccggggg
>>>>>>>>>>>>>>>>>>>>
61 tata8161ctacggtcaatgctctgaaatctgtggagcaaaccacagtttcatgcccatc

121 gtcctaga8221attaattcccctaaaaatctttgaaatagggcccgtatttaccctata

181 gcaccccctcta8281ccccctctagagcccactgtaaagctaacttagcattaaccttt

241 taagttaaagattaag8341agaaccaacacctctttacagtgaaatgccccaactaaat

301 actaccgtatggcccaccat8401aattacccccatactccttacactattcctcatcac

361 ccaactaaaaatattaaacacaaa8461ctaccacctacctccctcaccaaagcccataa
<<<<<<<<<<<<<<<<<<
421 aaataaaaaattataacaaaccctgaga8521accaaaatgaacgaaaatctgttcgctt
<<
481 cattcattgcccccacaatcctaggcctaccc

急请高手评价一下？非常感谢！！！！！！