Shotgun DNA Sequencing

When the Human Genome Mapping Project began in earnest, the allocation of large funding and the opening of dedicated research facilities promised much for the rapid improvement of DNA sequencing methods and, as a consequence, sequencing rates and throughput. Today, several years later, some novel approaches to the problem are being developed, such as sequencing by hybridization, and sequence detection using mass spectrometry and biochips, but these are far from in general use.