流式细胞技术检测法

 

<center> <div> <div> <div> <div> <table> <tbody> <tr> <td> <div> <p>  </p> <p> <strong><span><span></span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><b><span>1. 3</span> </b> <b><span>流式细胞技术检测法</span> </b> </span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span> </span></span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>原理：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>包绕着鞘液的单细胞或微粒经过流式细胞仪检测区域时被激发光激发出荧光信号，形成的不同波长荧光信号被光电倍增管接收后转换为电信号并进一步转换成计算机可识别的数字信号。其应用范围广泛，常用于细胞生物学、细胞遗传学、免疫学、肿瘤学、血液学等领域。</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>应用：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>1</span> <span>）了解细胞大小及颗粒度</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>2</span> <span>）细胞状态：<span>pH </span> 值、氧化还原、氧自由基、<span>Ca²⁺ </span> </span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>3</span> <span>）细胞表面蛋白：<span>CD </span> 系列等</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>4</span> <span>）细胞内蛋白：胞内因子、<span>P53</span> 等</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>5</span> <span>）细胞功能检测：细胞周期、凋亡 、分化、细胞增殖活力、吞噬功能、药物泵的功能<span> </span> </span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>7</span> <span>）免疫表型分析</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>流程：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>    </span> 1</span> <span>）根据实验需要预处理待检测细胞</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>2</span> <span>）上机<a href="https://www.biomart.cn/supply/10100139/" target="_blank">检测相关</a>参数</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>3</span> <span>）对所得结果进行数据分析</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>4</span> <span>）细胞分选流程（略）</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>基础背景：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>l<span>        </span> </span> </span> <span>提供背景资料，包括蛋白的表达位置，如是否膜蛋白或胞浆蛋白等</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>l<span>        </span> </span> </span> <span>需要检测的细胞，每种样本提供的细胞数量要大于<span>5×10⁵ </span> </span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span><span>l<span>        </span> </span> </span> <span>连接有或可连接荧光染料的一抗，并表明来源和应用浓度</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>我们提供：</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>详细的实验结果原始图及数据分析报告；所分选的细胞</span> </span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span> </span></span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span> </span></span></span></strong></p> <p class="MsoNormal"> <strong><span><span><span>http://www.research-bio.com/technology.asp?id=198&title=细胞技术服务</span> </span></span></strong></p> <strong><span></span></strong> <p>  </p> <p>  </p> </div> </td> </tr> </tbody> </table> </div> </div> </div> </div> </center>