【请教】用哪个免疫基因芯片好？
丁香园论坛
4824
博士课题要检测机体免疫功能方面的变化，其中部分使用基因芯片做，在网上查到有以下几种免疫相关基因芯片，不知道哪个更能反映机体的免疫功能改变，有高手指点一下吗？该用哪个基因芯片？
1。免疫信号通路基因芯片（395个基因）
免疫信号通路基因芯片包含了介导免疫应答和炎症的信号转导通路中的重要基因。芯片上的信号转导通路包括：NF-κB信号通路、Calcium/NFAT信号通路、MAP Kinase信号通路、JAK/STAT信号通路、PI3-AKT信号通路、及Toll-like受体信号通路。芯片上包含的基因可编码上述信号通路中的粘附分子、受体、配体、接头蛋白、调控因子、转录因子、激酶及下游效应蛋白。信号转导在免疫细胞激活、增殖、分化和凋亡中扮演了重要的角色，而这些生物学过程也与许多重要的病理及生理免疫反应有关，如：抗病毒反应、自身免疫、过敏症、炎症，及免疫缺陷。
基因列表(Functional Gene Grouping):
AKT and PI3K Family Members and Their Regulators:
AKT1, AKT2, AKT3, APPL, BTK, C20orf97, CTMP, GNB1, GRB2, GRB10, HSPB1, HSPCA, HSPCB, ILK, IMPDH1, INPP5D, INPPL1, MTCP1, PDK2, PDPK1, PIK3CA, PIK3CB, PIK3CG, PIK3R1, PIK3R2, PIK3R3, PPP2CA, PPP2CB, PPP2R1A, PPP2R1B, PPP2R2A, PPP2R2B, PPP2R2C, PPP2R3A, PPP2R4, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, PTEN, PRKCA, PRKCB1, PRKCZ, TCL1A, TCL1B.
JAK and STAT Proteins:
JAK1, JAK2, JAK3, STAT1, STAT2, STAT3, STAT4, STAT5A, STAT5B, STAT6, TYK2.
MAPK:
MAPK1, MAPK3, MAPK6, MAPK7, MAPK8, MAPK9, MAPK10, MAPK11, MAPK12, MAPK13, MAPK14.
MKK:
MAP2K1, MAP2K2, MAP2K3, MAP2K4, MAP2K5, MAP2K6, MAP2K7.
MKKK:
ARAF1, BRAF, DLK1, MAP3K1, MAP3K2, MAP3K3, MAP3K4, MAP3K5, MAP3K7, MAP3K11, MAP3K14, MAP4K1, MAP4K3, MOS, MST1, PAK1, PAK3, RAF1.
NFAT Family Members:
NFATC1, NFATC2, NFATC3, NFATC4, NFAT5.
Rel / NFkB / IkB Family:
NFKB1, NFKB2, NFKBIA, NFKBIB, NFKBIL1, NFKBIL2, NFRKB, REL, RELA, RELB.
Adhesion Molecules:
ICAM1, ICAM2, ICAM3, ICAM4, ICAM5, NCAM1, NOS2A, SELE, SELL, SELPLG, VCAM1.
Calcium Signaling Molecules:
CALM1, CALM2, CALM3, CAMK2B, CAMK4, CNR1, ITK, MAP2K7, NCK2, PAK1, PPIA, PPP3CB, PPP3CC, PPP3R1, TRPV6, VAV1, VAV2, VAV3.
Ligands:
CCL2, CSF2, CSF3, IFNA1, IFNB1, IFNG, IL1A, IL1B, IL2, IL3, IL4, IL6, IL8, IL10, IL12A, IL12B, IL13, IRF1, LTA, TGFB1, TNF, TNFSF5, TNFSF6, TNFSF11, VEGF.
Receptors:
CD3E, CD3G, CD3Z, CD69, CNR1, CSF1R, CSF2RB, EDG1, EGFR, EPOR, FCER1A, FCER2, FCGR1A, FCGR3A, IFNAR1, IFNAR2, IFNGR1, IFNGR2, IL1R1, IL1R2, IL2RA, IL2RG, IL4R, IL6ST, IL8RA, IL10RA, IL10RB, IL20RA, IL22RA1, MPL, NOTCH1, OPRD1, P2RX7, PTPRC, RIPK1, TNFRSF6, TNFRSF11A, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10.
Signal Transduction Kinases:
AKT1, AKT2, AKT3, CHUK, CSNK2A1, CSNK2B, FRAP1, GSK3A, GSK3B, IKBKB, IKBKG, IKBKE, MAP3K7IP1, PRKCA, PRKCB1, PRKCZ, RPS6KB1, SGK, SGKL, TBK1.
Nuclear Factors:
HMGA1, KPNA5, KPNB3, NCOA1, NMI, NUP214, SH2B, SPI1, SRC, STAM, STUB1, USF1, XPO5, YY1.
Transcription Factors:
ATF2, CEBPB, CREB1, CREBBP, EGFR, EGR1, EGR2, EGR3, ELK1, ELK3, ELK4, EP300, ETS1, ETS2, FKBP1B, FLJ14639 (NIP45), FOS, FOSL1, FOXO1A, FOXO3A, GATA3, GATA4, GRLF1, ICOS, IRF1, ISGF3G, JUN, JUNB, MADH4, MAPKAPK2, MAPKAPK3, MAX, MEF2A, MEF2B, MEF2C, MEF2D, MHC2TA, MKNK1, MLLT7, MYC, MYF5, NFKB2, NFKBIB, NFKBIE, NR4A2, PCNA, RAF1, RELA, RPS6KA5, SP1, SP3, TP53.
Adaptor Proteins:
FADD, IRAK1, IRAK2, MAP2K1IP1, MDM2, MYD88, SLA, TNFAIP3 (A20), TRADD, TRAF1, TRAF2, TRAF3, TRAF4, TRAF5, TRAF6.
Regulatory Proteins:
A2M, CABIN1, CASP3, CASP9, CDC37, CDC42, CFLAR, CTLA4, CRKL, GAB2, GNG2, GRB2, HRAS, INDO, KRAS2, KSR, MADH1, MADH2, MADH3, MADH4, MADH5, MADH6, MADH7, MADH9, MAPK8IP1, MCM5, MIZ1, NRAS, OSM, PAK1, PAK3, PIAS1, PIAS3, PIASY, PIM1, PREX1, PTPN1, PTPNS1, PTPRC, RAC1, RAC2, SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS7, SUGT1, TSC1, TSC2.
Response Proteins:
AGT, BAD, BCL2L1, BCL2L11, BF, C3, COL1A1, CRP, CSN2, CXCL9, DUSP1, EIF4EBP1, ENPP2, FN1, G1P2, G1P3, GATA3, GBP1, HSPA5, HSPB1, IRS1, MAF, MMP3, MST1, NPPB, NOS3, OAS1, ORM1, PIN1, SAA1, SFN, SLC2A4, SRF, TERT, YWHAZ.
Cell Cycle Proteins Regulated by the Erk1/2 Pathway:
CCNA1, CCNA2, CCNB1, CCNB2, CCND1, CCND2, CCND3, CCNE1, CCNE2, CDK2, CDK4, CDK6, CDKN1A, CDKN1B, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, E2F1, RB1.
2。T细胞与B细胞活化基因芯片
T细胞与B细胞活化基因芯片列有适应性免疫的关键组成－T细胞活化和B细胞活化的相关基因。休眠的淋巴细胞活化激发一系列复杂而有序的反应网络，最终产生适应性免疫。一个极端的表现为，过度活化这一精密和谐的网络系统，导致多种自体免疫疾病如系统性硬皮病、系统性红斑狼疮和自身免疫性肝炎。另外一种极端表现为，肿瘤细胞无法活化T淋巴细胞和B淋巴细胞是多种肿瘤的病源学基础。免疫疗法（即调控这一网络系统的方法入手）也许可以提供适应性免疫相关的多种疾病的治疗方法。此芯片可用来比对实验样本间的T细胞活化基因和B细胞活化基因的表达差异。
基因列表(Functional Gene Grouping):
B-cell activation:
Antigen dependent B-cell activation:
CD28, CD4, CD80, HLA-DRA, IL10, IL2, IL4, TNFRSF5, TNFRSF6, TNFSF5, TNFSF6.
Other genes involved in B-cell activation:
BLR1, HDAC4, HDAC5, HDAC7A, HDAC9, ICOSL, IGBP1, MS4A1, RGS1, SLA2.
B-cell proliferation:
CD81, IFNB1, IL10, TNFRSF5, TNFRSF7, TNFSF5.
B-cell differentiation:
AICDA, BLNK, GALNAC4S-6ST, HDAC4, HDAC5, HDAC7A, HDAC9, IL10, IL11, IL4, INHA, INHBA, KLF6, TNFRSF7.
T-cell activation:
Regulators of T-cell activation:
CD2, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, CD8A, CD8B1, CLECSF12, ICOSL, IRF4, KIF13B, NCK1, NCK2, PRLR, SIT, SLA2, TNFSF14.
T-cell proliferation:
CD28, CD3E, GLMN, ICOSL, IL10, IL12B, IL18, IL27, NCK1, NCK2, SFTPD, SPP1, TNFSF14.
T-cell differentiation:
CD1D, CD2, CD4, CD80, CD86, IL12B, IL2, IL27, IRF4, JAG2, NOS2A, RHOH, SOCS5, TNFRSF7, WWP1.
Regulators of Th1 and Th2 development:
ANPEP, CD2, CD33, CD5, CD7, CSF2, IFNA2, IFNB1, IFNG, IL10, IL12A, IL13, IL3, IL4, IL5, ITGAX, TLR2, TLR4, TLR7, TLR9, TNFRSF5.
Genes involved in Th1/Th2 differentiation:
CD28, CD86, HLA-DRA, IFNG, IFNGR1, IFNGR2, IL12A, IL12B, IL12RB1, IL12RB2, IL18, IL18R1, IL2, IL2RA, IL4, IL4R, PVRL1, TNFRSF5, TNFSF5.
Genes involved in T-cell polarization:
CCL3, CCL4, CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CD28, CD4, CSF2, CXCR3, CXCR4, IFNG, IFNGR1, IFNGR2, IL12A, IL12RB1, IL12RB2, IL18R1, IL2, IL4, IL4R, IL5, TGFB1, TNFSF5.
Other genes related to immune cell activation:
Macrophage activation:
C1QR1, IL31RA, INHA, INHBA, TLR1, TLR4, TLR6.
Neutrophil activation:
APOA2, IL8, PREX1, PRG3.
Natural killer cell activation:
CD2, IFNB1, IFNK, IL12B, IL2, IL21R, KIR3DL1, ULBP1, ULBP2, ULBP3.
Others:
AZU1, CX3CL1, ITIH1, TOLLIP, TXNDC, ZNF3.
3。血液学/免疫学基因芯片（440个基因）
血细胞是免疫系统的必要组成成分，在免疫功能中（如抗微生物反应，炎性反应和自身免疫反应）扮演重要角色，血液学/免疫学基因芯片用于研究血液学和免疫学中相关基因表达模型。芯片上包括配体基因（如细胞因子、趋化因子、生长因子），表面受体基因（如，细胞因子和趋化因子受体、造血细胞和免疫细胞标表面标志），信号转导分子基因（如受体蛋白、蛋白激酶）以及转录因子和细胞外基质分子基因。此芯片可用于研究血细胞和其它免疫系统细胞在细胞发展、分化，细胞系分化决定，细胞活化和增殖，细胞与ECM之间的信息传递和相互作用这些过程中的基因表达变化。
基因列表(Functional Gene Grouping):
B-Cell Surface Molecules (7):
CD80, CR1, CR2, HLA-DRA, ICAM1, ITGAL, PTPRC, CD19, CD86.
T-Cell Surface Molecules (9):
CD2, CD3D, CD3E, CD3G, CD3Z, CD4, ICAM1, ITGAL, PTPRC.
Cell Adhesion (43):
BBS2, CCL11, CCL2, CCL4, CCL5, CCR1, CCR3, CCR8, CD2, CD22, CD33, CD34, CD44, CD58, CD6, CD9, CSF3R, CX3CL1, CX3CR1, CXCL12, CXCR3, ENG, ICAM1, ICAM2, ICAM3, IL8, ITGA2, ITGA2B, ITGAE, ITGAL, ITGAX, ITGB2, ITGB3, ITGB7, KITLG, MLLT4, NK4, PTPRF, RAC1, SELL, SELP, TYRO3, VCAM1.
Cell Homeostasis and Motility (18):
BLR1, CCL4, CCL5, CCR6, CD9, CXCL10, CXCR3, IFNG, IL13, IL8, IL8RB, ITGAL, LSP1, MAP2K1, MCL1, RAC1, SELL, STAT3.
Blood Coagulation and Circulation (12):
APOB, CXCL10, CXCL12, DIA1, ENG, F10, IL10RB, IL20RA, ITGA2, ITGB3, PLAUR, WAS.
Platelet Activation (3):
ADAMTS13, CD9, IL11.
Complement Activation (11):
BF, C2, C3, C4A, C8A, C8B, CR1, CR2, DAF, HF1, IF.
Hematopoietin/Interferon-Class (D200-domain) Cytokine Receptor Activity (19):
CSF2RB, CSF3R, IFNAR2, IL10RA, IL10RB, IL12B, IL12RB1, IL12RB2, IL13RA1, IL20RA, IL2RB, IL3RA, IL4R, IL5RA, IL6R, IL6ST, IL7R, IL9R, LIFR.
Interleukin Receptors (8):
IL12RB1, IL12RB2, IL15RA, IL18R1, IL1R1, IL1R2, IL1RL1, IL1RL2.
Chemokine Receptors (17):
CCL13, CCR1, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CCRL1, CX3CR1, CXCR3, CXCR4, GPR17, GPR2, IL8RA, XCR1.
Antigen Binding and Processing (6):
CD1D, HLA-A, HLA-C, HLA-DRA, IL7R, LAG3.
B-Cell Activation (7):
BLR1, IL10, IL11, IL4, MS4A1, RGS1, TNFRSF7.
T-Cell Activation and Differentiation (24):
CD1D, CD2, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, HLA-DRA, IFNG, IL10, IL12A, IL12B, IL12RB1, IL12RB2, IL18, IL18R1, IL2, IL27, IL2RA, IL4, IL4R, IRF4.
Chemokines and Cytokines (80):
CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3L1, CCL4, CCL5, CCL7, CCL8, CSF3, CX3CL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL14, CXCL2, CXCL3, CXCL5, CXCL6, CXCL9, FLT3LG, GRN, IFNG, IL10, IL11, IL12A, IL12B, IL13, IL15, IL16, IL17, IL17B, IL17C, IL18, IL19, IL1A, IL1B, IL1F10, IL1F5, IL1F6, IL1F7, IL1F8, IL1F9, IL2, IL20, IL21, IL22, IL24, IL26, IL3, IL4, IL5, IL6, IL7, IL8, IL9, LIF, LTB, MIF, PPBP, THPO, TNF, TNFSF4, TNFSF7, TNFSF8, XCL1.
Other Hematology/Immunology Genes (246):
ABCB4, ABCB7, ABCC1, ABL1, ACK1, ADA, ADPRT, AF1Q, AGA, ALAD, ALK, ATF4, ATF7, ATM, ATRX, AXL, BATF, BAX, BCL2, BCL2A1, BCL2L1, BCL6, BCR, BIRC7, BLK, BLM, BPI, BSG, BTG1, BTK, CAMLG, CBFA2T1, CBL, CCL14, CCL22, CD14, CD19, CD1A, CD1B, CD1C, CD37, CD48, CD5, CD53, CD69, CD79A, CD79B, CD81, CD83, CEBPG, CES1, CHS1, CLC, CMRF35, CREB1, CSF1R, CTCF, CTLA4, CTSG, CXCR6, CYBB, DAPK1, DCC, DDX6, DEK, DHFR, DKC1, DNTT, DPP4, EBI2, EFNA1, ELL, ELL2, ENPP1, EPS15, EPX, ERG, ETS1, ETV6, EVI2A, EWSR1, FAH, FCAR, FCER2, FCGR3A, FECH, FES, FGF6, FGFR1, FLI1, FLT4, FOXK2, FOXP3, FTH1, FUS, FVT1, FY, GATA1, GATA2, GATA3, GPR18, GSTP1, GZMA, GZMH, HBB, HCLS1, HHEX, HIVEP1, HLF, HOXA4, HOXA5, HOXB6, HOXD3, HOXD4, HTLF, ICSBP1, ID2, IFI16, IL11RA, IL17R, IL18RAP, IL1RN, IL21R, IL23A, IL27RA, ILF2, ILF3, IRAK1, IRF1, IRF2, ITK, JAK3, LAF4, LCK, LCP1, LCP2, LMO1, LMO2, LYL1, LYN, MAD, MAL, MAP2K3, MAP2K4, MAP2K6, MAPK9, MATK, MDM2, MGAM, MHC2TA, MLL, MLL2, MLL3, MLL5, MLLT1, MLLT10, MLLT3, MLLT6, MLLT7, MME, MNDA, MPO, MVP, MYH11, NFATC1, NFATC3, NFIX, NF-ΚB2, NME3, NOTCH1, NPM1, NTRK1, NUP214, PAX5, PBX1, PBX2, PBX3, PDGFA, PHF11, PICALM, PIM1, POU2AF1, POU2F1, POU2F2, POU3F1, POU5F1, PRF1, PRTN3, PTGIR, PTPN11, RAC2, RAG1, RARA, RBMS1, RFX1, RGS2, RHOH, RIPK1, RUNX1, RUNX3, RYR1, S100A12, SDS, SERPINB2, SF1, SFTPD, SH2D1A, SLC11A1, SLPI, SPI1, STAT1, STAT2, STAT5B, STAT6, STMN1, SYK, TAL1, TAZ, TBX1, TCF12, TCF3, TCF7, TCF7L1, TCF7L2, TCF8, TGFB2, TGFBR3, TIAL1, TLX1, TM4SF2, TNFRSF17, TNFRSF8, TNK1, TOP1, TXK, TYMS, VAV2, VHL, WEE1, WT1, YWHAQ, ZAP70, ZNF145, ZNF42, ZNF593, ZNFN1A1.
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1。免疫信号通路基因芯片（395个基因）
免疫信号通路基因芯片包含了介导免疫应答和炎症的信号转导通路中的重要基因。芯片上的信号转导通路包括：NF-κB信号通路、Calcium/NFAT信号通路、MAP Kinase信号通路、JAK/STAT信号通路、PI3-AKT信号通路、及Toll-like受体信号通路。芯片上包含的基因可编码上述信号通路中的粘附分子、受体、配体、接头蛋白、调控因子、转录因子、激酶及下游效应蛋白。信号转导在免疫细胞激活、增殖、分化和凋亡中扮演了重要的角色，而这些生物学过程也与许多重要的病理及生理免疫反应有关，如：抗病毒反应、自身免疫、过敏症、炎症，及免疫缺陷。
基因列表(Functional Gene Grouping):
AKT and PI3K Family Members and Their Regulators:
AKT1, AKT2, AKT3, APPL, BTK, C20orf97, CTMP, GNB1, GRB2, GRB10, HSPB1, HSPCA, HSPCB, ILK, IMPDH1, INPP5D, INPPL1, MTCP1, PDK2, PDPK1, PIK3CA, PIK3CB, PIK3CG, PIK3R1, PIK3R2, PIK3R3, PPP2CA, PPP2CB, PPP2R1A, PPP2R1B, PPP2R2A, PPP2R2B, PPP2R2C, PPP2R3A, PPP2R4, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, PTEN, PRKCA, PRKCB1, PRKCZ, TCL1A, TCL1B.
JAK and STAT Proteins:
JAK1, JAK2, JAK3, STAT1, STAT2, STAT3, STAT4, STAT5A, STAT5B, STAT6, TYK2.
MAPK:
MAPK1, MAPK3, MAPK6, MAPK7, MAPK8, MAPK9, MAPK10, MAPK11, MAPK12, MAPK13, MAPK14.
MKK:
MAP2K1, MAP2K2, MAP2K3, MAP2K4, MAP2K5, MAP2K6, MAP2K7.
MKKK:
ARAF1, BRAF, DLK1, MAP3K1, MAP3K2, MAP3K3, MAP3K4, MAP3K5, MAP3K7, MAP3K11, MAP3K14, MAP4K1, MAP4K3, MOS, MST1, PAK1, PAK3, RAF1.
NFAT Family Members:
NFATC1, NFATC2, NFATC3, NFATC4, NFAT5.
Rel / NFkB / IkB Family:
NFKB1, NFKB2, NFKBIA, NFKBIB, NFKBIL1, NFKBIL2, NFRKB, REL, RELA, RELB.
Adhesion Molecules:
ICAM1, ICAM2, ICAM3, ICAM4, ICAM5, NCAM1, NOS2A, SELE, SELL, SELPLG, VCAM1.
Calcium Signaling Molecules:
CALM1, CALM2, CALM3, CAMK2B, CAMK4, CNR1, ITK, MAP2K7, NCK2, PAK1, PPIA, PPP3CB, PPP3CC, PPP3R1, TRPV6, VAV1, VAV2, VAV3.
Ligands:
CCL2, CSF2, CSF3, IFNA1, IFNB1, IFNG, IL1A, IL1B, IL2, IL3, IL4, IL6, IL8, IL10, IL12A, IL12B, IL13, IRF1, LTA, TGFB1, TNF, TNFSF5, TNFSF6, TNFSF11, VEGF.
Receptors:
CD3E, CD3G, CD3Z, CD69, CNR1, CSF1R, CSF2RB, EDG1, EGFR, EPOR, FCER1A, FCER2, FCGR1A, FCGR3A, IFNAR1, IFNAR2, IFNGR1, IFNGR2, IL1R1, IL1R2, IL2RA, IL2RG, IL4R, IL6ST, IL8RA, IL10RA, IL10RB, IL20RA, IL22RA1, MPL, NOTCH1, OPRD1, P2RX7, PTPRC, RIPK1, TNFRSF6, TNFRSF11A, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10.
Signal Transduction Kinases:
AKT1, AKT2, AKT3, CHUK, CSNK2A1, CSNK2B, FRAP1, GSK3A, GSK3B, IKBKB, IKBKG, IKBKE, MAP3K7IP1, PRKCA, PRKCB1, PRKCZ, RPS6KB1, SGK, SGKL, TBK1.
Nuclear Factors:
HMGA1, KPNA5, KPNB3, NCOA1, NMI, NUP214, SH2B, SPI1, SRC, STAM, STUB1, USF1, XPO5, YY1.
Transcription Factors:
ATF2, CEBPB, CREB1, CREBBP, EGFR, EGR1, EGR2, EGR3, ELK1, ELK3, ELK4, EP300, ETS1, ETS2, FKBP1B, FLJ14639 (NIP45), FOS, FOSL1, FOXO1A, FOXO3A, GATA3, GATA4, GRLF1, ICOS, IRF1, ISGF3G, JUN, JUNB, MADH4, MAPKAPK2, MAPKAPK3, MAX, MEF2A, MEF2B, MEF2C, MEF2D, MHC2TA, MKNK1, MLLT7, MYC, MYF5, NFKB2, NFKBIB, NFKBIE, NR4A2, PCNA, RAF1, RELA, RPS6KA5, SP1, SP3, TP53.
Adaptor Proteins:
FADD, IRAK1, IRAK2, MAP2K1IP1, MDM2, MYD88, SLA, TNFAIP3 (A20), TRADD, TRAF1, TRAF2, TRAF3, TRAF4, TRAF5, TRAF6.
Regulatory Proteins:
A2M, CABIN1, CASP3, CASP9, CDC37, CDC42, CFLAR, CTLA4, CRKL, GAB2, GNG2, GRB2, HRAS, INDO, KRAS2, KSR, MADH1, MADH2, MADH3, MADH4, MADH5, MADH6, MADH7, MADH9, MAPK8IP1, MCM5, MIZ1, NRAS, OSM, PAK1, PAK3, PIAS1, PIAS3, PIASY, PIM1, PREX1, PTPN1, PTPNS1, PTPRC, RAC1, RAC2, SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS7, SUGT1, TSC1, TSC2.
Response Proteins:
AGT, BAD, BCL2L1, BCL2L11, BF, C3, COL1A1, CRP, CSN2, CXCL9, DUSP1, EIF4EBP1, ENPP2, FN1, G1P2, G1P3, GATA3, GBP1, HSPA5, HSPB1, IRS1, MAF, MMP3, MST1, NPPB, NOS3, OAS1, ORM1, PIN1, SAA1, SFN, SLC2A4, SRF, TERT, YWHAZ.
Cell Cycle Proteins Regulated by the Erk1/2 Pathway:
CCNA1, CCNA2, CCNB1, CCNB2, CCND1, CCND2, CCND3, CCNE1, CCNE2, CDK2, CDK4, CDK6, CDKN1A, CDKN1B, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, E2F1, RB1.
2。T细胞与B细胞活化基因芯片
T细胞与B细胞活化基因芯片列有适应性免疫的关键组成－T细胞活化和B细胞活化的相关基因。休眠的淋巴细胞活化激发一系列复杂而有序的反应网络，最终产生适应性免疫。一个极端的表现为，过度活化这一精密和谐的网络系统，导致多种自体免疫疾病如系统性硬皮病、系统性红斑狼疮和自身免疫性肝炎。另外一种极端表现为，肿瘤细胞无法活化T淋巴细胞和B淋巴细胞是多种肿瘤的病源学基础。免疫疗法（即调控这一网络系统的方法入手）也许可以提供适应性免疫相关的多种疾病的治疗方法。此芯片可用来比对实验样本间的T细胞活化基因和B细胞活化基因的表达差异。
基因列表(Functional Gene Grouping):
B-cell activation:
Antigen dependent B-cell activation:
CD28, CD4, CD80, HLA-DRA, IL10, IL2, IL4, TNFRSF5, TNFRSF6, TNFSF5, TNFSF6.
Other genes involved in B-cell activation:
BLR1, HDAC4, HDAC5, HDAC7A, HDAC9, ICOSL, IGBP1, MS4A1, RGS1, SLA2.
B-cell proliferation:
CD81, IFNB1, IL10, TNFRSF5, TNFRSF7, TNFSF5.
B-cell differentiation:
AICDA, BLNK, GALNAC4S-6ST, HDAC4, HDAC5, HDAC7A, HDAC9, IL10, IL11, IL4, INHA, INHBA, KLF6, TNFRSF7.
T-cell activation:
Regulators of T-cell activation:
CD2, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, CD8A, CD8B1, CLECSF12, ICOSL, IRF4, KIF13B, NCK1, NCK2, PRLR, SIT, SLA2, TNFSF14.
T-cell proliferation:
CD28, CD3E, GLMN, ICOSL, IL10, IL12B, IL18, IL27, NCK1, NCK2, SFTPD, SPP1, TNFSF14.
T-cell differentiation:
CD1D, CD2, CD4, CD80, CD86, IL12B, IL2, IL27, IRF4, JAG2, NOS2A, RHOH, SOCS5, TNFRSF7, WWP1.
Regulators of Th1 and Th2 development:
ANPEP, CD2, CD33, CD5, CD7, CSF2, IFNA2, IFNB1, IFNG, IL10, IL12A, IL13, IL3, IL4, IL5, ITGAX, TLR2, TLR4, TLR7, TLR9, TNFRSF5.
Genes involved in Th1/Th2 differentiation:
CD28, CD86, HLA-DRA, IFNG, IFNGR1, IFNGR2, IL12A, IL12B, IL12RB1, IL12RB2, IL18, IL18R1, IL2, IL2RA, IL4, IL4R, PVRL1, TNFRSF5, TNFSF5.
Genes involved in T-cell polarization:
CCL3, CCL4, CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CD28, CD4, CSF2, CXCR3, CXCR4, IFNG, IFNGR1, IFNGR2, IL12A, IL12RB1, IL12RB2, IL18R1, IL2, IL4, IL4R, IL5, TGFB1, TNFSF5.
Other genes related to immune cell activation:
Macrophage activation:
C1QR1, IL31RA, INHA, INHBA, TLR1, TLR4, TLR6.
Neutrophil activation:
APOA2, IL8, PREX1, PRG3.
Natural killer cell activation:
CD2, IFNB1, IFNK, IL12B, IL2, IL21R, KIR3DL1, ULBP1, ULBP2, ULBP3.
Others:
AZU1, CX3CL1, ITIH1, TOLLIP, TXNDC, ZNF3.
3。血液学/免疫学基因芯片（440个基因）
血细胞是免疫系统的必要组成成分，在免疫功能中（如抗微生物反应，炎性反应和自身免疫反应）扮演重要角色，血液学/免疫学基因芯片用于研究血液学和免疫学中相关基因表达模型。芯片上包括配体基因（如细胞因子、趋化因子、生长因子），表面受体基因（如，细胞因子和趋化因子受体、造血细胞和免疫细胞标表面标志），信号转导分子基因（如受体蛋白、蛋白激酶）以及转录因子和细胞外基质分子基因。此芯片可用于研究血细胞和其它免疫系统细胞在细胞发展、分化，细胞系分化决定，细胞活化和增殖，细胞与ECM之间的信息传递和相互作用这些过程中的基因表达变化。
基因列表(Functional Gene Grouping):
B-Cell Surface Molecules (7):
CD80, CR1, CR2, HLA-DRA, ICAM1, ITGAL, PTPRC, CD19, CD86.
T-Cell Surface Molecules (9):
CD2, CD3D, CD3E, CD3G, CD3Z, CD4, ICAM1, ITGAL, PTPRC.
Cell Adhesion (43):
BBS2, CCL11, CCL2, CCL4, CCL5, CCR1, CCR3, CCR8, CD2, CD22, CD33, CD34, CD44, CD58, CD6, CD9, CSF3R, CX3CL1, CX3CR1, CXCL12, CXCR3, ENG, ICAM1, ICAM2, ICAM3, IL8, ITGA2, ITGA2B, ITGAE, ITGAL, ITGAX, ITGB2, ITGB3, ITGB7, KITLG, MLLT4, NK4, PTPRF, RAC1, SELL, SELP, TYRO3, VCAM1.
Cell Homeostasis and Motility (18):
BLR1, CCL4, CCL5, CCR6, CD9, CXCL10, CXCR3, IFNG, IL13, IL8, IL8RB, ITGAL, LSP1, MAP2K1, MCL1, RAC1, SELL, STAT3.
Blood Coagulation and Circulation (12):
APOB, CXCL10, CXCL12, DIA1, ENG, F10, IL10RB, IL20RA, ITGA2, ITGB3, PLAUR, WAS.
Platelet Activation (3):
ADAMTS13, CD9, IL11.
Complement Activation (11):
BF, C2, C3, C4A, C8A, C8B, CR1, CR2, DAF, HF1, IF.
Hematopoietin/Interferon-Class (D200-domain) Cytokine Receptor Activity (19):
CSF2RB, CSF3R, IFNAR2, IL10RA, IL10RB, IL12B, IL12RB1, IL12RB2, IL13RA1, IL20RA, IL2RB, IL3RA, IL4R, IL5RA, IL6R, IL6ST, IL7R, IL9R, LIFR.
Interleukin Receptors (8):
IL12RB1, IL12RB2, IL15RA, IL18R1, IL1R1, IL1R2, IL1RL1, IL1RL2.
Chemokine Receptors (17):
CCL13, CCR1, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CCRL1, CX3CR1, CXCR3, CXCR4, GPR17, GPR2, IL8RA, XCR1.
Antigen Binding and Processing (6):
CD1D, HLA-A, HLA-C, HLA-DRA, IL7R, LAG3.
B-Cell Activation (7):
BLR1, IL10, IL11, IL4, MS4A1, RGS1, TNFRSF7.
T-Cell Activation and Differentiation (24):
CD1D, CD2, CD3D, CD3E, CD3G, CD4, CD7, CD80, CD86, HLA-DRA, IFNG, IL10, IL12A, IL12B, IL12RB1, IL12RB2, IL18, IL18R1, IL2, IL27, IL2RA, IL4, IL4R, IRF4.
Chemokines and Cytokines (80):
CCL1, CCL11, CCL13, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3L1, CCL4, CCL5, CCL7, CCL8, CSF3, CX3CL1, CXCL10, CXCL11, CXCL12, CXCL13, CXCL14, CXCL2, CXCL3, CXCL5, CXCL6, CXCL9, FLT3LG, GRN, IFNG, IL10, IL11, IL12A, IL12B, IL13, IL15, IL16, IL17, IL17B, IL17C, IL18, IL19, IL1A, IL1B, IL1F10, IL1F5, IL1F6, IL1F7, IL1F8, IL1F9, IL2, IL20, IL21, IL22, IL24, IL26, IL3, IL4, IL5, IL6, IL7, IL8, IL9, LIF, LTB, MIF, PPBP, THPO, TNF, TNFSF4, TNFSF7, TNFSF8, XCL1.
Other Hematology/Immunology Genes (246):
ABCB4, ABCB7, ABCC1, ABL1, ACK1, ADA, ADPRT, AF1Q, AGA, ALAD, ALK, ATF4, ATF7, ATM, ATRX, AXL, BATF, BAX, BCL2, BCL2A1, BCL2L1, BCL6, BCR, BIRC7, BLK, BLM, BPI, BSG, BTG1, BTK, CAMLG, CBFA2T1, CBL, CCL14, CCL22, CD14, CD19, CD1A, CD1B, CD1C, CD37, CD48, CD5, CD53, CD69, CD79A, CD79B, CD81, CD83, CEBPG, CES1, CHS1, CLC, CMRF35, CREB1, CSF1R, CTCF, CTLA4, CTSG, CXCR6, CYBB, DAPK1, DCC, DDX6, DEK, DHFR, DKC1, DNTT, DPP4, EBI2, EFNA1, ELL, ELL2, ENPP1, EPS15, EPX, ERG, ETS1, ETV6, EVI2A, EWSR1, FAH, FCAR, FCER2, FCGR3A, FECH, FES, FGF6, FGFR1, FLI1, FLT4, FOXK2, FOXP3, FTH1, FUS, FVT1, FY, GATA1, GATA2, GATA3, GPR18, GSTP1, GZMA, GZMH, HBB, HCLS1, HHEX, HIVEP1, HLF, HOXA4, HOXA5, HOXB6, HOXD3, HOXD4, HTLF, ICSBP1, ID2, IFI16, IL11RA, IL17R, IL18RAP, IL1RN, IL21R, IL23A, IL27RA, ILF2, ILF3, IRAK1, IRF1, IRF2, ITK, JAK3, LAF4, LCK, LCP1, LCP2, LMO1, LMO2, LYL1, LYN, MAD, MAL, MAP2K3, MAP2K4, MAP2K6, MAPK9, MATK, MDM2, MGAM, MHC2TA, MLL, MLL2, MLL3, MLL5, MLLT1, MLLT10, MLLT3, MLLT6, MLLT7, MME, MNDA, MPO, MVP, MYH11, NFATC1, NFATC3, NFIX, NF-ΚB2, NME3, NOTCH1, NPM1, NTRK1, NUP214, PAX5, PBX1, PBX2, PBX3, PDGFA, PHF11, PICALM, PIM1, POU2AF1, POU2F1, POU2F2, POU3F1, POU5F1, PRF1, PRTN3, PTGIR, PTPN11, RAC2, RAG1, RARA, RBMS1, RFX1, RGS2, RHOH, RIPK1, RUNX1, RUNX3, RYR1, S100A12, SDS, SERPINB2, SF1, SFTPD, SH2D1A, SLC11A1, SLPI, SPI1, STAT1, STAT2, STAT5B, STAT6, STMN1, SYK, TAL1, TAZ, TBX1, TCF12, TCF3, TCF7, TCF7L1, TCF7L2, TCF8, TGFB2, TGFBR3, TIAL1, TLX1, TM4SF2, TNFRSF17, TNFRSF8, TNK1, TOP1, TXK, TYMS, VAV2, VHL, WEE1, WT1, YWHAQ, ZAP70, ZNF145, ZNF42, ZNF593, ZNFN1A1.
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