We describe a suspension array hybridization assay for rapid detection and identification of Salmonella and other bacterial pathogens using Luminex� xMAP™ technology. The Luminex xMAP system allows simultaneous detection of up to 100 different targets in a single multiplexed rea ...
The study of Salmonella spp. encompasses numerous disciplines and a wide range of specialties. Salmonella is exceptional among microorganisms in that it is capable of causing many common intestinal illnesses and yet has great potential as a molecular tool in the fight against disease.
Physical mapping is a key methodology for determining the genome structure of Salmonella and revealing genomic differences among different strains, especially regarding phylogenetic relationships and evolution of these bacteria. In fact, physical mapping is the only practi ...
Salmonellae are mammalian pathogens that are transmitted mainly through foodstuffs and their handlers. Rapid detection requires both specificity and sensitivity in samples containing other bacteria. A solution to this problem is the use of the great specificity conferred by bact ...
A simple macroarray system based on the use of polyester cloth as the solid phase for DNA hybridization has been developed for the identification and characterization of bacteria on the basis of the presence of various virulence and toxin genes. In this approach, a multiplex polymerase chain re ...
Pathogenicity islands and genomic islands (GI) are key elements in the evolution of bacterial virulence and environmental adaptation. In Salmonella enterica, Salmonella pathogenicity islands (SPI) confer important virulence traits; however, many of these loci have not been cha ...
Microarray technology provides a convenient and relatively inexpensive way of investigating the genetic content of bacterial genomes by comparative genomic hybridization. In this method, genomic DNA of an unknown bacterial strain of interest and that of a closely related sequenc ...
Amplified fragment length polymorphism (AFLP) is a powerful PCR-based fingerprinting method and has the capacity to reveal variation around the whole genome by selectively amplifying a subset of restriction fragments for comparison. The restriction fragments analyzed are sma ...
The performance of many bacterial genetic experiments would benefit from a convenient method to clone large sets of genes (20–100+kb) and transfer these genes to a wide range of other bacterial recipients. The VEX-Capture technique allows such large genomic segments to be cloned in vivo onto a b ...
Numerous bacteriophages specific to Salmonella have been isolated or identified as part of host genome sequencing projects. Phylogenetic analysis of the sequenced phages, based on related protein content using CoreGenes, reveals that these viruses fall into five groupings (P27- ...
Henry Ford thought that hlstory was bunk; Hegel is supposed to have said that “people and governments never have learnt anything from history,” and Disraeli advised us to “read no history.” The authors of this chapter are less dismissive, taking the alternative view that those who ignore history are ...
Neurological disorders impose a severe burden on affected individuals Inherited neurological disorders of late onset present their own specific difficulties in terms of genetic counseling, management, and patients’ psychological adaptation. Huntington’s disease (HD) is t ...
Scrapie, as a disease of sheep and goats, has been recognized for over 300 yr and was first documented in England in 1730 (1). Sheep breeders petitioned the House of Commons of King George II in 1754 requesting the enforcing of regulations governing the sale and distribution of sheep This is believed to have ...
The basis of strain vartation in scrapie and other transmissible spongrform encephalopathies is a crucial issue in the ongoing debate about the nature of the infectious agent. The clear evidence for the existence of multiple strains leads us to conclude that these agents carry some form of str ...
The Transmissible Spongiform Encephalopathies (TSEs), such as scrapre, BSE, and Creutzfeldt-Jakob disease, are associated with alterations in the neural membrane protein or prion protein (PrP). This chapter will outline the gene targeting approaches that have been used to mutate the ...
Like conventrinal viruses, prion isolates exhibit distinctive, and often restricted host-ranges. However, the molecular events that shape the hostranges of these two classes of pathogen are dissimilar, reflecting their fundamentally different life cycles. As discussed by Rid ...
The pathogenesis of scrapie and other transmissible spongiform encephalopathies (TSEs) appears to be based on the posttranslational conversion of the host’s protease-sensitive prion protein (PrP-sen or PrPc) to abnormal protease-resistant forms (PrP-res or PrPSc). In vitro st ...
Prion encephalopathies are neurodegenerative diseases characterized by the accumulation of abnormal isoforms of the prion protein (PrP) and the deposition of PrP amyloid in the central nervous systein (CNS) (1, 2) The diseasespecific PrP molecules are distinguishable froin their ...
Viral diseases were largely untreatable 40 yr ago. Now effective and safe therapies are available. This has led to significant improvements in the quality of life for large numbers of patients. New viral diseases are, however, continuing to emerge and established viruses have been shown to dev ...
In the laboratory it is important that potentially pathogenic agents be controlled to protect the laboratory worker from infection and the experiment from contamination. The operation of a safe laboratory depends on many factors: the training and judgement of laboratory personnel; t ...
