丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Amplified Fragment Length Polymorphism Analysis of Salmonella enterica

互联网

638
Amplified fragment length polymorphism (AFLP) is a powerful PCR-based fingerprinting method and has the capacity to reveal variation around the whole genome by selectively amplifying a subset of restriction fragments for comparison. The restriction fragments analyzed are small, and even mutation of 1 bp can be detected. The use of different sets of restriction enzymes or different primer combinations can generate large numbers of different AFLP fingerprints. AFLP is of particular value for studies of closely related strains, such as analysis of variation within a serovar of Salmonella enterica . We present here protocols for both radioactively labeled and fluorescent dye-labeled AFLP analyses that are also applicable to other bacterial species. Fluorescent AFLP has proved to be reproducible and capable of standardization.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序