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丁香实验推荐阅读
A Clinically Relevant Thromboembolic Stroke Model in the Aged Rat

Animal models have been an integral component of scientific discovery concerning ischemic stroke pathophysiology and the accompanying therapeutic targets. Unfortunately, the knowledge gleaned from these studies has failed to translate to enhanced therapeutics in the clin ...

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Use of Cell-Stretch System to Examine the Characteristics of Mechanosensor Channels: Axonal Growth/Neuroregeneration Studies

Temperature-sensitive TRP (so-called “thermoTRP”) channels are well recognized for their contributions to sensory transduction, responding to a wide variety of stimuli including temperature, nociceptive stimuli, touch, and osmolarity. However, the precise roles for the the ...

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Methods in Neuronal Growth Cone Biology

Axon guidance is a crucial component of normal nervous system development. Errors in axon guidance can result in disorders such as autism and schizophrenia. In order to understand the pathogenesis of these disorders, we need to understand the fundamental mechanisms of growth cone motili ...

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TRP Channels in the Brain

In the past few years the presence of one or another member of the TRP channel family has been described basically in all tissues studied. Moreover, the pattern of expression and the susceptibility to modulators of each channel subtype in each tissue will shape their influence on the cell/tissue re ...

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Investigation of the Possible Role of TRP Channels in Schizophrenia

Schizophrenia is a debilitating psychiatric disorder. The limitations of current treatments for schizophrenia have led to an ongoing search for new drug targets. The observations that subjects with schizophrenia have impaired thermoregulation, are less sensitive to pain than n ...

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Investigating Diseases of Dopaminergic Neurons and Melanocytes Using Zebrafish

Zebrafish offer experimental advantages that have been exploited by developmental biologists and, �increasingly, by those interested in disease mechanisms. Parkinson’s disease is characterized by the loss of dopaminergic neurons of the substantia nigra and motor symptoms su ...

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Transient Receptor Potential Channels and Pruritus

Itch induction and allergy development are regulated by highly complex pathways that involve dynamic cross-talk events between cells within the skin. These include the transient receptor potential (TRP) ion channels. For example, the intensity, quality, and the clinical characte ...

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Skin Sensitivity Studies

Sensitive skin is defined as the occurrence of erythema and/or abnormal stinging, burning, and tingling sensations (and sometimes as pain or pruritus) in response to multiple factors that may be physical (UV, heat, cold, wind), chemical (cosmetics, soaps, water, pollutants), psychologic ...

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Rodent Models of Conditioned Fear: Behavioral Measures of Fear and Memory

Pavlovian fear conditioning is a robust technique for examining behavioral and cellular components of fear learning and memory. In fear conditioning, the subject learns to associate a previously neutral stimulus with an inherently noxious co-stimulus. The learned association is r ...

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A Practical Guide to Evaluating Anxiety-Related Behavior in Rodents

Tests of innate fear and anxiety take advantage of the natural anxieties and interests of mice and rats. They often rely on the animals’ motivation to explore their environment as well as their apprehension towards novelty or exposure. Here we describe the technical and experimental details of ...

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Balloon Injury in Rats as a Model for Studying TRP Channel Contribution to Vascular Smooth Muscle Remodeling

Many vascular occlusive diseases are characterized by endothelial dysfunction and phenotypic switch of vascular smooth muscle cell (VSMC) from quiescent contractile to proliferative migratory phenotypes. These cellular responses can be recapitulated and studied in vivo in ...

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Models of Hypertension and Blood Pressure Recording

Hypertension and cardiovascular events continue to constitute the leading global diseases affecting around 20% of the world’s population. Although there have been significant advances in the treatment and prevention of these diseases, important efforts are being made aiming at de ...

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Atherosclerosis Models with Cell-Mediated Calcification

This protocol describes a novel chronic total occlusion (CTO) model that features cell-mediated calcium deposits in rabbit femoral arteries. CTO is the most severe case of atherosclerosis, and it remains a big challenge in cardiology. There are urgent needs to establish a CTO animal model in o ...

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Methods to Study the Effects of TRP Channel Drugs on Vascular Endothelial Cell Function

Transient receptor potential (TRP) channels are abundantly expressed in vascular endothelium where the channels play important functional roles. In this chapter, we describe some well-established approaches, from molecular detection to functional assays, to study the role of T ...

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Anemic Zebrafish Models of Cardiomyopathy

Cardiomyopathy refers to the cardiac remodeling process in response to a variety of intrinsic and extrinsic stimuli that stress the heart. To discover novel therapeutic strategies for the disease, we are establishing and characterizing adult zebrafish models of cardiomyopathy. O ...

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TRPs to Cardiovascular Disease

Transient receptor potential (TRP) is a large superfamily of cation channels comprising 28 members in mammals. TRP channels are ubiquitously expressed in human tissues, including the cardiovascular system where they have been associated with a number of physiological functions, s ...

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Animal Models of Muscular Dystrophy

Muscular dystrophy is a severe degenerative disorder of the skeletal muscle, characterized by progressive muscle weakness. One subgroup of this disease is caused by a defect in the genes encoding the components of the dystrophin–glycoprotein complex. Such a defect results in a signific ...

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Collagen Antibody-Induced Arthritis: A Disease-Relevant Model for Studies of Persistent Joint Pain

Rheumatoid arthritis (RA) is a complex autoimmune disease, with a prevalence of approximately 1% in the population worldwide. RA is a chronic inflammatory disease primarily affecting joints and patients suffer from a plethora of symptoms, including pain, fatigue and stiffness. Joint p ...

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Methods for the Assessment of Heat Perception in Humans

Several transient receptor potential (TRP) ion channels are activated by heat and may potentially �contribute to human heat perception though clinical data are currently either limited or absent. At least three of the identified heat-activated TRP channels are appealing drug targe ...

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Methods to Study Thermonociception in Rodents

The study of thermonociception in conscious animals is based on the assessment of pain-avoiding behavior from noxious heat or cold stimuli. The conventional tests of heat nociception apply suprathreshold heat stimuli and measure the reflex latency of withdrawal reactions. A novel ap ...

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