Cloning in Plasmid Vectors

A fundamental step in molecular biology is the cloning of a DNA fragment insert into a plasmid vector. This allows the cloned fragment to be replicated upon transformation of the recombinant molecule into a bacterial cell (see Chapters 4 and 5 ) so that the DNA of interest can be investigated further. Cloning is an essential part of many experiments, including library generation (see Chapter 18 ) and expression studies (see Chapter 29 ).