Discovery and Customized Validation of Antibody Targets by Protein Arrays and Indirect ELISA

Because of our access to human genome data and ever improving genome sequencing and proteome analysis methods we are much better in terms of our understanding of biological processes. In addition to genomics, proteomics, and other “omics” methods, availability of more sophisticated molecular assaying methods have augmented our knowledge about immune processes towards autogeneic and allogeneic targets. High-density protein arrays are developed to analyze protein–small molecule interactions, enzyme–substrate profiling, protein–protein interaction, and immune monitoring by assessing antibodies in the serum.