Visualizing Nuclear Structure In Situ by Atomic Force Microscopy

The cell nucleus is an organelle where molecules involved in gene expression are highly compartmentalized (1 ) in very dynamic (2 ) territories. This current notion of functional organization has been possible because of many studies of this organelle that included its cell and molecular organization, and where microscopy played an important role. Light and electron microscopy in conjunction with molecular approaches, such as the construction of both antibodies and nucleic acid probes, as well as molecular construction of genes with green fluorescent protein and fluorescence recovery after photobleaching technology, in addition to high-resolution in situ hybridization and immunocy-tochemistry now offer a more complete knowledge.