Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low-Water Media

A simple immobilization method for Candida rugosa lipase on superparamagnetic Fe₃ O₄ nanoparticles is described. The Fe₃ O₄ nanoparticles were coated with PEI and Candida rugosa lipase was adsorbed on these particles via electrostatic interactions. The immobilization resulted in marginal simultaneous purification. However, the immobilized preparation showed 110� higher transesterification activity in low-water media. It was also efficient in kinetic resolution of (�)-1-phenylethanol with ee_p of 99 % and E = 412 within 24 h.