MicroRNA Profiling of Megakaryocytes
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MiRNAs are non-coding RNAs of 19–25 nucleotides in length that regulate gene expression. Recent work suggests a role for miRNAs during hematopoiesis. Here, we described in detail how to successfully obtain highly pure megakaryocytes precursors from human CD34+ stem cells in culture to perform high-throughput miRNA profiling using a custom microarray platform.
