BLOTTING W/ BSA
互联网
1328
- Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt
- Wash 2X for 5' in 1XPBST
-
10Ab in 1% BSA/1X PBST --60'/Rt
-
Wash PBST 15', then 3x for 5' each in 1X PBST
-
20Ab in 1% BSA/1X PBST --60'/Rt (aHRP)--
-
Wash 15' with 1X PBST then 3x with PBST for 5' @
- Chemiluminescence: Mix 1:1 NEN reagents. Add to Mb --1'/shaking. Blot excess
- Put between sheets (page protector) and expose film
上一篇:WESTERNS 下一篇:SILVER STAIN OF PROTEIN GELS