BLOTTING W/ BSA
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1300
- Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt
- Wash 2X for 5' in 1XPBST
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10Ab in 1% BSA/1X PBST --60'/Rt
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Wash PBST 15', then 3x for 5' each in 1X PBST
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20Ab in 1% BSA/1X PBST --60'/Rt (aHRP)--
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Wash 15' with 1X PBST then 3x with PBST for 5' @
- Chemiluminescence: Mix 1:1 NEN reagents. Add to Mb --1'/shaking. Blot excess
- Put between sheets (page protector) and expose film
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