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DNA甲基化分析

互联网

1530

 

The influence of methylation on the promoter activity and gene expression and the involvement of DNA methylation in carcinogenesis caused an extensive research of methylation processes and it's influence on the genome functioning. Analysis of DNA methylation became a routine method during the last decade. I worked with methylation extensively during my PhD studies and tried several methods. Here I place examples of 3 main approaches for methylation analysis. All these methods I have used personally, therefore if you have any questions don't hesitate to contact me.


 

Methylation analysis using restriction enzyme digestion PDF

 

Contributed by A. Gratchev

Protocol

 

Contributed by A.Gratchev

Protocol

 

Contributed by A.Gratchev

Protocol for 32 P labelled nucleotides

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