Isolation of Mouse Thymic Dendritic Cells
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The method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (1 ,2 ) and involves the following major steps:
| 1. |
Enzymatic digestion of thymic fragments with collagenase and DNase.
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| 2. |
Separation of a very-low-density cell fraction (VLDF).
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| 3. |
Magnetic depletion of T-lineage cells, B cells, macrophages, and granulocytes.
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| 4. |
Positive selection of DCs by magnetic cell sorting (MACS).
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