Endothelial Cell Migration Under Flow

The endothelial cells lining blood vessels are continuously exposed to fluid shear stress generated by pulsatile flow of blood. In order to minimise forces acting on their surface, endothelial cells adapt to shear stress by alignment and migration within the direction of flow. Failure to adapt to shear stress results in endothelial damage contributing to generation of atherosclerotic plaques or abnormal vessel repair. This chapter describes methods of generating laminar flow in vitro and studying endothelial cell alignment and motility under flow.