IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detection
互联网
Solutions:
- Hydrogen peroxide
- SIGMA Catalog No. H-1009 (5 ml)
- Proteinase K
- Dissolve Proteinase K (Sigma Catalog No. P-4914) in dH2O for a concentration of 20mg/ml.
- 1% BSA/PBS
- 1g Fraction V BSA in 100ml 1x PBS. Mix in a beaker with low heat. Aliquot in 15ml tubes and store at -20°C.
- Hematoxylin
- 75ml Gill's hematoxylin no.2 in 75ml dH2O.
- 1% Acid-alcohol
- 2ml HCl in 198ml of 70% ethanol.
- Scott's Solution
- NaHCO3, 2g
- MgSO4.7H2O, 20g
- dH2O, 1000ml