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引物延伸反应

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Primer extension analysis is used to determine the location and quantitate the amount of the 5´-end of specific RNAs. An end-labeled oligonucleotide is hybridized to RNA and is utilized as a primer by reverse transcriptase in the presence of deoxynucleotides. The RNA is thus reverse transcribed into cDNA, which is analyzed on a denaturing polyacrylamide gel. The length of the cDNA reflects the number of bases between the labeled nucleotide of the primer and the 5´-end of the RNA; the quantity of cDNA product is proportional to the amount of targeted RNA

         Primer Extension (Promega)

         Primer Extension (Bowtell Lab)
Including procedures for primer labeling and  primer extension assay.

         Primer Extension (Petra Klaff)

         Pol II in vitro Transcription and Primer Extension Assay (Hahn Lab)

         Primer Extension of Total Yeast RNA (Hahn Lab)

         Primer Labeling for Primer Extension Assay and Primer Extension (PMCI Research)

 

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