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Cell and tissue lysis hub

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This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison. The ups and downs of various methods from sonication, homogenization, freeze-thaw cycles, and detergent-based lysis are examined below.

Comparison of lysis methods

 

Sonication

material: ultrasonication bath or rods

 

Homogenisation

material: drills (polytron), pestle/tube (dounce) bead beaters

 

Freeze-thaw

material: pestle and mortar, liquid nitrogen

 

Detergents

no additional material required, just the chemicals and typical tubes

 

Specific protocols

 

target    lysis method    type of material   
Silver: Lysate for Western protein detergent (Triton) cells
Sauer:Lysing E. coli with Lysozymes   lysozyme bacteria
Blackburn:Yeast Colony PCR DNA NaOH yeast
Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer protein detergent (RIPA buffer) cells
Streptomyces:Protocols/Mini-Maxi Prep DNA NaOH bacteria
Western Blot/Tissue Preparation protein detergent & physical disruption tissue
Eccles:Protein Lysates from Cells in Culture protein detergent (SDS, NP40) cells
Eccles:Protein Lysates from Tissue protein detergent, physical, freezing tissue
RNA extraction using trizol/tri RNA phenol cells
Sauer:RNA Purification from E. coli RNA various options bacteria
add another method''s name target lysis method type of starting material

 

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