【求助】miRNA的反义下调方面问题
丁香园论坛
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各位师兄，小弟做miRNA 的microdowm，但realtime检测antisence转染后，miRNA的表达并没用下调，问试剂公司的人说 antisence不能下调目的miRNA的表达，只有上调的质粒才能影响miRNA的表达，是这样吗，多谢！！
我个人认为公司说的不太准确。利用反义核酸或siRNA抑制microRNA表达是一个很成熟的技术，当然效果如何取决于当初反义核酸或siRNA设计时是否得当，一般需要设计3－5条片段，从中选择效果最好的进行后续实验。
too many factors will affect your result.
generally the efficiency of antisense technology is not that high. also, the design of the oligo is critical.
other things like the transfection efficiency of your cells, the time of your treatment etc...
It is really hard to say anything if you don't have positive control.
generally the efficiency of antisense technology is not that high. also, the design of the oligo is critical.
other things like the transfection efficiency of your cells, the time of your treatment etc...
It is really hard to say anything if you don't have positive control.
非常感谢！！！！！！！！！！！！！
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