Clone Excision Methods for the Lambda ZAP-Based Vectors

The Lambda ZAP� vectors have been designed to allow in vivo excision and recircularization of the cloned insert and phagemid sequences contained within the λ vector (1 ). Once a Lambda ZAP library is constructed and amplified, putative clones or the λ library itself may be excised into the phagemid form. Two versions of the excision protocol for Lambda ZAP-based vectors are included here.