Real-Time PCR Fluorescent Chemistries

There are more than a dozen formats available for the fluorescent detection of amplified DNA in kinetic (real-time) PCR. These chemistries are adaptable to most real-time PCR instruments and may offer benefits over the usual manufacturer-recommended chemistries for the instrument. The most popular chemistries are the generic dye, SYBR Green I, TaqMan�, and hybridization probes. However, there are now new dyes being reported with superior fluorescent detection and product resolution; as well as new probe formats that may offer advanced multiplexing opportunities for quantification and genotyping.