Incubation of Fear
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- Abstract
- Table of Contents
- Materials
- Figures
- Literature Cited
Abstract
While fear and anxiety can grow over time in anxiety disorders, most efforts to model this phenomenon with fear conditioning in rodents cause fear that remains stable or decreases across weeks or months. Here, we describe several methods to induce conditioned fear that grows over the course of 1 month and is sustained for at least 2 months using an extended fear conditioning approach. These methods include a very reliable standard method that causes multiple fear measures to increase over months, as well as alternative methods. Curr. Protoc. Neurosci. 64:6.27.1?6.27.11. © 2013 by John Wiley & Sons, Inc.
Keywords: fear incubation; fear conditioning; delayed?onset fear; PTSD; anxiety
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PDF or HTML at Wiley Online LibraryTable of Contents
- Introduction
- Basic Protocol 1: Concurrent Freezing/Conditioned Suppression Incubation with Short Cues
- Alternate Protocol 1: Fear/Anxiety Incubation with Long Cues
- Alternate Protocol 2: Fear Incubation Measured Without a Concurrent Operant Task
- Commentary
- Literature Cited
- Figures
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Basic Protocol 1: Concurrent Freezing/Conditioned Suppression Incubation with Short Cues
Materials
Alternate Protocol 1: Fear/Anxiety Incubation with Long Cues
Materials
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Figure 6.27.1 Time‐line for the and . View Image -
Figure 6.27.2 Time‐course of fear incubation using the procedure described in the Basic Protocol. The measure of fear is conditioned suppression of lever‐pressing (mean + SEM). View Image -
Figure 6.27.3 Comparison of fear expression after limited (1 day) or extended (10 day) training. (A ) Fear expression after training with 30‐sec cues (as in the Basic Protocol). (B ) Fear expression after training with 6‐min cues (as in ). The measure of fear is conditioned suppression of lever‐pressing (mean + SEM). View Image -
Figure 6.27.4 Different trial‐by‐trial patterns of fear incubation (idealized data). (A ) Higher fear expression on day 31 on every trial during the fear test. (B ) Higher fear expression on day 31 early in the test with the differences disappearing on later trials. (C ) Similar early in the test due to ceiling effects, with fear expression on day 31 higher on later trials. View Image
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Literature Cited
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| Internet Resources | |
| http://www.pickenslab.net | |
| Web site for the Pickens laboratory (Kansas State University) starting in August 2013. This site contains sample programs for all three protocols. |
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