骨组织的荧光染色
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Some fluorescent compounds, which are fixed in newly formed calcified tissue, are used to label bone deposition. Such labels help to determine the time sequence of bone growth. The label may be given single or double label by intraperitoneal injection.
|
Agents
|
Sigma Cat #
|
Color
|
Dosage (mg/kg)
|
Stock Con. (mg/ml)
|
Diluents
|
| Calcein |
C-0875
|
Green
|
10
|
3
|
2%NaHCO3
PH7.4 |
| Xylenaol Orange(XO) |
X-0127
|
Red
|
90
|
30
|
2%NaHCO3
PH7.4 |
| Alizarin Complexone(AC) |
A-3882
|
Red
|
30
|
10
|
2%NaHCO3
PH7.4 |
| Oxytetracycline Hydrochloride(OH) |
O-5875
|
Yellow
|
30
|
10
|
20% EtOH
|
|
Age
|
First Injection Before Sacrificing (days)
|
Second Injection Before Sacrificing (days)
|
| <3 months |
10
|
2
|
| >3 months, <6 months |
15
|
5
|
| > 6 months |
20
|
8
|
