CD34分子
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CD34 分子
CD34
常用单克隆抗体或代号： MY10,ICH3
主要表达细胞： BM,En [M]
分子质量(kDa)和结构： gp115(与Ig- SFC2组一定相似性)
功 能： 调控早期造血,为CD62L的配体,外周淋巴结地址素,外周淋巴结粘附
CD34
Cell-cell adhesion molecule and cell surface glycoprotein
May mediate attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells
Antibodies are used to quantify and purify lymphohemaoietic stem cell / progenitor cells for research and for clinical bone marrow transplantation; however, counting CD34+ mononuclear cells may overestimate myeloid blasts in bone marrow smears due to hematogones and CD34+ megakaryocytes, Archives 2002;126:823
Interpretation: membranous stain; endothelium acts as a positive internal control
Uses: possibly confirm chronic intestinal pseudo-obstruction, AJSP 2003;27:228
Positive staining (normal): hemaoietic progenitor cells, vascular endothelial cells (these cells are TdT+, HLA-DR+)
Positive staining (disease): alveolar soft part sarcoma, preB-ALL (75%), AML (40%), AML-M7 (most), chordoid gliomas, dendritic fibromyxolipoma, dermatofibrosarcoma protuberans, epithelioid sarcomas, gastrointestinal stromal tumors, giant cell fibroblastoma, granulocytic sarcoma, hamartomatous tumors of chronic drug resistant epilepsy, hemangiopericytomas (50%), inflammatory fibrous polyps of stomach (Hum Path 2002;33:307), Kaposi’s sarcoma (strong, diffuse in spindle cells), liposarcomas (myxoid, pleomorphic), MFH, malignant peripheral nerve sheath tumors, meningeal hemangiopericytomas, meningiomas (some), metanephric adenosarcoma of kidney (stroma only), myelodysplastic syndrome (myeloid blasts), myofibroblastoma of breast, neurofibromas, nuchal fibromas (also CD99+), papillary thyroid carcinoma, paratesticular leiomyosarcoma (30%), pleomorphic hyalinizing angiectatic tumor, pseudoangiomatous stromal hyperplasia of breast, schwannomas (usually), sclerosing liposarcoma (spindle and atypical cells), solitary fibrous tumors, synovial sarcoma (focal in 6% of monophasic tumors), blasts in transient myeloproliferative disorder
Negative staining: Ewing’s sarcoma/PNET, myofibrosarcoma of breast, inflammatory myofibroblastic tumors of stomach
Micro images: granulocytic sarcoma, malignant solitary fibrous tumor, GIST and solitary fibrous tumor, GIST (figure B), CD34+ blasts and megakaryoctyes, cavernous hemangioma of spermatic cord, solitary fibrous tumor of orbit
Micro images (AJSP subscribers): hybrid DFSP-giant cell fibroblastoma, solitary fibrous tumor of kidney, metanephric adenosarcoma of kidney (stroma only), rectal GIST (figure B), paratesticular leiomyosarcoma, normal small bowel (figure 1B), normal colon (figure 1B), chronic intestinal pseudo-obstruction (figure 1B), Gardner associated fibromas, microcystic adnexal carcinoma A, B
Micro images (Mod Path subscribers): GIST (figures C, D)
Reference: Archives 2002;126:1189, AJSP 2003;27:27, AJSP 2002;26:932, AJSP 2001;25:1451, AJSP 2001;25:1194; AJSP 2001;25:645, AJSP 2001;25:464, Mod Path 2002;15:125
