求引物设计
丁香园论坛
1205
刚准备作RT-RCR， 自己设计了一个引物，烦劳各位专家指点评价
mmp-2 rat
基因编码
1 cgggtggacc ccagggcact gccacgacct cagggtgaca cgcggagccc gggagcgcaa
61 ggatggaggc acgattggtc tggggagtgc tcgtcggacc tctcagggtt ctctgcgtcc
121 tgtgctgcct gctgggccag gtggacccca gggcactgcc acgacctcag ggtgacacgc
181 ggagcccggg agcgcaagga tggaggcacg attggtctgg ggagtgctcg tcggacctct
241 cagggttctc tgcgtcctgt gctgcctgct gggcgggtgg accccagggc actgccacga
301 cctcagggtg acacagcgag cccgggagcg caaggatggg aggcacgatt ggtctgggga
361 gtgctcgtcg gacctctcag ggttctctgc gtcctgtgct gcctgcttgg gcgggtggac
421 cccagggcac tgccacgacc tcagggtgac acgcggagcc cgggagcgca aggatggagg
481 cacgattggt ctggggagtg ctcgtcggac ctctcagggt tctctgcgtc ctgtgctgcc
541 tgctgggcca cgccatcgct gcaccatcgc ccatcatcaa gttccccggc gatgtcgccc
601 ctaaaacaga caaagagttg gcagtgcaat acctgaatac tttctatggc tgccccaagg
661 agagttgcaa cctctttgtg ctgaaagata ccctcaagaa gatgcagaag ttctttgggc
721 tgccccagac aggtgacctt gaccagaaca ccatcgagac catgcggaag ccaagatgtg
781 gcaacccaga tgtggccaac tacaacttct tcccccgcaa gcccaagtgg gacaagaacc
841 agatcacata caggatcatt ggttacacac ctgacctgga ccctgaaacc gtggatgatg
901 cttttgctcg ggccttaaaa gtatggagcg acgtcactcc gctgcgcttt tctcgaatcc
961 atgatgggga ggctgacatc atgatcaact ttggacgctg ggagcatgga gatggatacc
1021 cgtttgatgg caaggatgga ctcctggcac atgcctttgc cccgggcact ggtgttgggg
1081 gagattctca ctttgatgat gatgagctgt ggaccctggg agaaggacaa gtggtccgcg
1141 taaagtatgg gaacgctgat ggcgagtact gcaagttccc cttcctgttc aacggtcggg
1201 aatacagcag ctgtacagac actggtcgca gtgatggctt cctctggtgc tccactacat
1261 acaactttga gaaggatggc aagtatggct tctgccccca tgaagccttg tttaccatgg
1321 gtggcaatgc tgatggacag ccctgcaagt ttccgttccg cttccagggc acctcctaca
1381 acagctgtac caccgagggc cgcaccgacg gctaccgctg gtgtggcacc accgaggact
1441 atgaccggga taagaaatat ggattctgcc ccgagaccgc tatgtccact gtgggtggaa
1501 attcagaagg tgccccatgt gtcttcccct tcactttcct gggcaacaag tatgagagct
1561 gcaccagcgc cggccgcaac gatggcaagg tgtggtgtgc aaccacaacc aactacgatg
1621 atgaccggaa gtggggcttc tgtcctgacc aaggatatag cctattcctc gtggcagccc
1681 atgagttcgg ccatgccatg gggctggaac actctcagga ccctggagct ctgatggccc
1741 ctatctacac ctacaccaag aacttccgat tatcccatga tgacatcaag gggatccagg
1801 agctctatgg gccctcccct gatgctgata ctgacactgg tactggcccc acaccgacat
1861 tgggacctgt cactccggag atctgcaaac aggacattgt ctttgatggc atcgctcaga
1921 tccgtggtga gatcttcttc ttcaaggacc ggtttatttg gcggacagtg acaccacgtg
1981 acaagcccac aggtcccttg ctggtggcca cattctggcc tgagctccca gaaaagattg
2041 acgctgtgta tgaggcccca caggaggaga aggctgtgtt cttcgcaggg aatgagtact
2101 gggtctattc tgccagcact ctggagcgag gataccccaa gccactgacc agcctggggt
2161 tgccccctga tgtccagcaa gtagatgctg cctttaactg gagtaagaac aagaagacat
2221 acatctttgc aggagacaag ttctggagat acaatgaagt gaagaagaaa atggacccgg
2281 gtttccctaa gctcatcgca gactcctgga atgccatccc tgataacctg gatgctgtcg
2341 tggacctgca gggcggtggt catagctact tcttcaaggg tgcttattac ctgaagctgg
2401 agaaccaaag tctcaagagc gtgaagtttg gaagcatcaa atcagactgg ctgggctgct
2461 gagctggccc tgttcccacg ggccctatca tcttcatcgc tgcacaccag gtgaaggatg
2521 tgaagcagcc tggcggctct gtcctcctct gtagttaacc agccttctcc ttcacctggt
2581 gacttcagat ttaagagggt ggcttctttt tgtgcccaaa gaaaggtgct gactgtaccc
2641 tcctgggtgc tgcttctcct tcctgcccac cctaggggat gcttggatat gtgcaatgca
2701 gccctactct gggctgccct ggtgctccac tcttctggtt cttcaacatc tatgaccttt
2761 tattggcttc agcactctca gagttaatag agactggctt aggagggcac tggtggccct
2821 gttaacagcc tggcatgggg cagtggggta caggtgtgcc aaggtggaaa tcagagacac
2881 ctggtttcac cctttctgat gcccagatac ctgcaccacc ttaactgttg ctttgtatgc
2941 cccttcgctc gtttccttca accttttcag ttttccactc cactgcattt cctgcccaaa
3001 ggactcgggt tgtctgacat cgctgcatga tgcatctcag cccgcctagt gatggttccc
3061 ctcctcactc tgtgcagtca tgcccagtca cttcctccac tggatggagg agaaccaagt
3121 cagtggcgtg gaaaactgaa aaggttgaag gaaacgagcg aagggcatac aaaagcaaca
3181 gttaaggtgg tgcaggtatc tgggcagcag aaagggtgaa accaggtggc a
引物序列
sense 5-CAT TGG TTA CAC ACC TGA CCT-3;
antisense primer, 5-CAG CGT TCC CAT ACT TTA CTC-3
mmp-2 rat
基因编码
1 cgggtggacc ccagggcact gccacgacct cagggtgaca cgcggagccc gggagcgcaa
61 ggatggaggc acgattggtc tggggagtgc tcgtcggacc tctcagggtt ctctgcgtcc
121 tgtgctgcct gctgggccag gtggacccca gggcactgcc acgacctcag ggtgacacgc
181 ggagcccggg agcgcaagga tggaggcacg attggtctgg ggagtgctcg tcggacctct
241 cagggttctc tgcgtcctgt gctgcctgct gggcgggtgg accccagggc actgccacga
301 cctcagggtg acacagcgag cccgggagcg caaggatggg aggcacgatt ggtctgggga
361 gtgctcgtcg gacctctcag ggttctctgc gtcctgtgct gcctgcttgg gcgggtggac
421 cccagggcac tgccacgacc tcagggtgac acgcggagcc cgggagcgca aggatggagg
481 cacgattggt ctggggagtg ctcgtcggac ctctcagggt tctctgcgtc ctgtgctgcc
541 tgctgggcca cgccatcgct gcaccatcgc ccatcatcaa gttccccggc gatgtcgccc
601 ctaaaacaga caaagagttg gcagtgcaat acctgaatac tttctatggc tgccccaagg
661 agagttgcaa cctctttgtg ctgaaagata ccctcaagaa gatgcagaag ttctttgggc
721 tgccccagac aggtgacctt gaccagaaca ccatcgagac catgcggaag ccaagatgtg
781 gcaacccaga tgtggccaac tacaacttct tcccccgcaa gcccaagtgg gacaagaacc
841 agatcacata caggatcatt ggttacacac ctgacctgga ccctgaaacc gtggatgatg
901 cttttgctcg ggccttaaaa gtatggagcg acgtcactcc gctgcgcttt tctcgaatcc
961 atgatgggga ggctgacatc atgatcaact ttggacgctg ggagcatgga gatggatacc
1021 cgtttgatgg caaggatgga ctcctggcac atgcctttgc cccgggcact ggtgttgggg
1081 gagattctca ctttgatgat gatgagctgt ggaccctggg agaaggacaa gtggtccgcg
1141 taaagtatgg gaacgctgat ggcgagtact gcaagttccc cttcctgttc aacggtcggg
1201 aatacagcag ctgtacagac actggtcgca gtgatggctt cctctggtgc tccactacat
1261 acaactttga gaaggatggc aagtatggct tctgccccca tgaagccttg tttaccatgg
1321 gtggcaatgc tgatggacag ccctgcaagt ttccgttccg cttccagggc acctcctaca
1381 acagctgtac caccgagggc cgcaccgacg gctaccgctg gtgtggcacc accgaggact
1441 atgaccggga taagaaatat ggattctgcc ccgagaccgc tatgtccact gtgggtggaa
1501 attcagaagg tgccccatgt gtcttcccct tcactttcct gggcaacaag tatgagagct
1561 gcaccagcgc cggccgcaac gatggcaagg tgtggtgtgc aaccacaacc aactacgatg
1621 atgaccggaa gtggggcttc tgtcctgacc aaggatatag cctattcctc gtggcagccc
1681 atgagttcgg ccatgccatg gggctggaac actctcagga ccctggagct ctgatggccc
1741 ctatctacac ctacaccaag aacttccgat tatcccatga tgacatcaag gggatccagg
1801 agctctatgg gccctcccct gatgctgata ctgacactgg tactggcccc acaccgacat
1861 tgggacctgt cactccggag atctgcaaac aggacattgt ctttgatggc atcgctcaga
1921 tccgtggtga gatcttcttc ttcaaggacc ggtttatttg gcggacagtg acaccacgtg
1981 acaagcccac aggtcccttg ctggtggcca cattctggcc tgagctccca gaaaagattg
2041 acgctgtgta tgaggcccca caggaggaga aggctgtgtt cttcgcaggg aatgagtact
2101 gggtctattc tgccagcact ctggagcgag gataccccaa gccactgacc agcctggggt
2161 tgccccctga tgtccagcaa gtagatgctg cctttaactg gagtaagaac aagaagacat
2221 acatctttgc aggagacaag ttctggagat acaatgaagt gaagaagaaa atggacccgg
2281 gtttccctaa gctcatcgca gactcctgga atgccatccc tgataacctg gatgctgtcg
2341 tggacctgca gggcggtggt catagctact tcttcaaggg tgcttattac ctgaagctgg
2401 agaaccaaag tctcaagagc gtgaagtttg gaagcatcaa atcagactgg ctgggctgct
2461 gagctggccc tgttcccacg ggccctatca tcttcatcgc tgcacaccag gtgaaggatg
2521 tgaagcagcc tggcggctct gtcctcctct gtagttaacc agccttctcc ttcacctggt
2581 gacttcagat ttaagagggt ggcttctttt tgtgcccaaa gaaaggtgct gactgtaccc
2641 tcctgggtgc tgcttctcct tcctgcccac cctaggggat gcttggatat gtgcaatgca
2701 gccctactct gggctgccct ggtgctccac tcttctggtt cttcaacatc tatgaccttt
2761 tattggcttc agcactctca gagttaatag agactggctt aggagggcac tggtggccct
2821 gttaacagcc tggcatgggg cagtggggta caggtgtgcc aaggtggaaa tcagagacac
2881 ctggtttcac cctttctgat gcccagatac ctgcaccacc ttaactgttg ctttgtatgc
2941 cccttcgctc gtttccttca accttttcag ttttccactc cactgcattt cctgcccaaa
3001 ggactcgggt tgtctgacat cgctgcatga tgcatctcag cccgcctagt gatggttccc
3061 ctcctcactc tgtgcagtca tgcccagtca cttcctccac tggatggagg agaaccaagt
3121 cagtggcgtg gaaaactgaa aaggttgaag gaaacgagcg aagggcatac aaaagcaaca
3181 gttaaggtgg tgcaggtatc tgggcagcag aaagggtgaa accaggtggc a
引物序列
sense 5-CAT TGG TTA CAC ACC TGA CCT-3;
antisense primer, 5-CAG CGT TCC CAT ACT TTA CTC-3
