Detection of Unstable Trinucleotide Repeats
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Accurate allele sizing is essential;
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Polymerase chain reaction (PCR) amplification across the repeat is hampered by extreme guanme cytosme (GC) content and strong secondary structure, and
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Size differences between normal and mutated alleles may be great, for instance, in fragile X they can range from 6 to over 1000 repeats
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