Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
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The cytogenetic analysis of single cells, such as oocytes and polar bodies, is extremely challenging. The main problem is
low probability of obtaining a metaphase preparation in which all of the chromosomes are sufficiently well spread to permit
accurate analysis (no overlapping chromosomes, no chromosomes lost). As a result, a high proportion of the oocytes subjected
to cytogenetic analysis are not suitable for traditional chromosome banding studies or for molecular cytogenetic methods such
as spectral karyotyping (SKY) or multiplex fluorescence in situ hybridization (M-FISH). Fortunately, recent innovations in
whole genome amplification and microarray technologies have provided a means to analyze the copy number of every chromosome
in single cells with high accuracy. Here we describe the use of such methods for the investigation of chromosome and chromatid
abnormalities in human oocytes and polar bodies.