Sl Nuclease Analysis of RNA

The analysis of RNA molecules by S1 nuclease digestion constitutes an important tool for molecular studies of cellular processes, not only because of the historical significance of its contribution to messenger ribonucleic acids (mRNA) structure analyses, but also for its simplicity and wide range of applications Following a brief introduction to important aspects of RNA studies, this chapter presents a detailed description of the theory and principles underlying the technique of RNA mapping by S1 -nuclease digestion, as well as a discussion of the most relevant applications of this molecular technique