Histochemical GUS Analysis

Patterns in the localization of compounds, metabolic processes, and regulatory machineries at the cellular and subcellular levels are studied by scientific disciplines, called cytochemistry (biochemistry of the cell, cellular topochemistry) or histochemistry, if the tissue level is concerned. In order to reach these goals, a whole range of procedures have been developed, which may be divided in two groups: the in vitro techniques, based on homogenate fractionation and the in situ techniques, realized on sections. Only in situ techniques are considered in this chapter (1 –5 ).