Immunogold Labeling of Resin-Embedded Electron Microscopical Sections

Knowledge about the spatio-temporal distribution patterns of proteins and other molecules of the cell is essential for understanding their function. A widely used technique is immunolabeling which uses specific antibodies to reveal the distribution of molecular components at various structural levels. Electron microscopy offers the highest resolution of morphological techniques and is thus an indispensable tool for the analysis of molecule distribution patterns at the subcellular level. In this chapter we describe a routine method for labeling ultrathin sections of resin-embedded material with antibodies conjugated to colloidal gold.