PCR from Tissue

 

1. collect piece of tissue (e.g., pieces of a leaf or flower) in Eppendorf tube containing 40 ml 0.25 N NaOH
2. put in boiling H₂ O for 30 sec (optimum may need to be determined for each type of tissue, for floral tissue 30 sec is fine)
3. neutralize by adding 40 ml 0.25 N HCl and 20 ml 0.5 M Tris-HCl pH 8.0, 0.25% (v/v) Igepal CA-630
4. boil for another 2 min
5. use directly for PCR or store at 4°C for up to several weeks
6. if stored at 4°C boil again for 2 min before the PCR

 

Remarks:

We don't have too much experience with this method. Mostly it seems to work though. However, I don't think it is safe enough for critical purposes (e.g., mapping).

PCR方法相关产品:

• 电泳设备
• 紫外设备
• 普通PCR仪
• 定量PCR仪
• PCR/RT-PCR/qPCR试剂
• PCR引物
• PCR试剂
• PCR对照
• 特异性PCR试剂盒
• PCR克隆试剂盒
• RNA
• RNase检测/去除
• RT-PCR试剂
• RT-PCR标准品
• 定量PCR试剂
• 定量PCR标记
• 总RNA分离纯化盒
• PCR产物纯化
• 核酸酶
• 聚合酶
• 反转录酶