RNA Purification Protocol for 100-200 mg of Animal Tissue
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实验原理
实验试剂
实验设备
1. Centrifuge capable of 3,000-5,000 x g
2. High speed centrifuge tube with capacity of at least 15 ml
实验步骤
3. Add 2 ml of PNP Buffer to the cell lysate. Mix the sample gently by inverting the tube 10 times.
4. Place the tube on ice for 5 minutes.
7. Gently mix the solution by inverting the tube 30-40 times.
8. Centrifuge at 3,000-5,000 x g for 15 minutes at room temperature.
11. Invert the tube on a clean adsorbent paper and air dry the pellet for 10-15 minutes.
12. Add 250 ul of DEPC Water and vortex for 1 minutes to mix.