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Methods for In Vivo and In Vitro Analysis of Innate Immune Responses to Helicobacter pylori Infection

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It is estimated that half of the world’s population is infected by Helicobacter pylori (H. pylori ) (Polk and Peek, Nat Rev Cancer 10:403–414, 2010; Peek et al., Physiol Rev 90:831–858, 2010). Following infection, H. pylori induces a chronic innate immune response that is thought to contribute to gastric complications. Due to the widespread prevalence of H. pylori , it is important to study the innate immune responses that result from the infection. A variety of in vitro and in vivo techniques have been developed by our laboratory to study this immune response (Fox et al., Am J Pathol 171:1520–1528, 2007; Kurt-Jones et al., Infect Immun 75:471–480, 2007; Kurt-Jones et al., J Endotoxin Res 10:419–424, 2004). These methods are described here.
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