Derivation of Murine ES Cell Lines

Embryonic stem (ES) cells have had a tremendous impact on the field of genetics and are widely used as a means for precise genetic modification of the mouse genome. This chapter will give a background to the use of these versatile cells and provide practical protocols for their derivation and culture. These techniques critically depend on attention to detail and quality control. We will thoroughly discuss potential pitfalls and provide additional techniques that can be used in cases where derivation has previously failed.