Liquid Chromatographic Analysis of Monoamines and Their Metabolites

It is evident that an explosive growth has occurred over the past ten years in the high performance liquid chromatographic (HPLC) analysis of catecholamines, indoleamines, and the trace amines In part, this expansion can be attributed to a growing interest in the monoamines; however, the increased sensitivity and specificrty afforded by the HPLC methods and their low cost must be viewed as the major reason for their wide acceptance. It is also clear that the vast preponderance of HPLC methods in neurochemistry utilize either amperometric or fluorometric detection systems. The bases of these methods of detection are well established and the benefits resulting from their couplmg to HPLC systems and their pervasive use in neurochemistry have been previously reviewed (Anderson and Young, 1981 , 1982 ; Davis and Kissinger, 1982, Kissinger et al., 1977 , 1981 ; Krstulovic, 1982 ; Mefford, 1981 , Warsh et al., 1982 ).