Fluorescence Microscopy Methods

The last decade has seen an extraordinary improvement in fluorescence microscopy instruments. These have been mainly in the development of epi-illumination systems, including dichroic beam splitters, matching excitation and barrier filters, and special objectives (see ref. 1 for background information). Routinely we use a JenaLumar fluorescence microscope (Zeiss, Jena, Germany) equipped with oil immersion wide field � 100 objective with an adjustable iris diaphragm. According to our experience, the best documentation is obtained when the photographs are taken on Kodak Tri X-Pan film processed in Kodak developer.