His-tag protein purification using Ni-NTA magnetic beads

互联网2013-09-06

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His-tag protein purification using Ni-NTA magnetic beads

Materials and reagents

Extraction buffer

<center> 20 mM Tris-HCl, pH 8.0 1 mM PMSF 1 mg/ml lysozyme 0.05% Triton X-100 </center>

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