His-tag protein purification using Ni-NTA magnetic beads
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His-tag protein purification using Ni-NTA magnetic beads
Materials and reagents
Extraction buffer
| <center> <font>20 mM Tris-HCl, pH 8.0 </font> <p> <font>1 mM PMSF</font></p> <p> <font>1 mg/ml lysozyme</font></p> <p> <font>0.05% Triton X-100</font></p> </center> |
