DNA concentration-Molecular Biology

Does somebody know how to increase DNA concentration from a too weakly concentrated DNA sample obtained with a Qiagen Extraction Kit (the elution buffer was ddH₂O) ??

-BATY-

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You should precipitate your DNA in 0.1V 3M NaOAc pH5.2 + 3V EtOH place -20℃ 30 min spin 15kxg wash pellet in 70% EtOH then resuspend in 10mM tris pH7.5 with a smaller volume than you started.

-milanoj-

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Elute with elution buffer warmed at 50

-Benoit-

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easy evaperate in 72℃

that is ok

-hello-

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Baty,

I didn't think of this before. The protocol specifically calls for elution in EB buffer (10 mM Tris pH 7.5). Check the pH of your ddH₂O. Usually it's pretty acidic and this will lead to low DNA concentrations upon elution.

-milanoj-

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