Arsonoliposomes: Preparation and Physicochemical Characterization

Arsonoliposomes (ARSL) which are liposomes that contain arsonolipids in their membranes have shown interesting anticancer and antiparasitic activity in vitro. Their lipid composition (the specific arsonolipids and/or phospholipids used for their preparation, and the relative amounts of each lipid type) highly influences their physicochemical properties as well as their in vivo kinetics and antiparasitic activity; however, their cytotoxicity towards cancer cells is minimally – if at all – modified. ARSL are prepared by a modification of the “one step” method followed or not by sonication (for formation of sonicated or non-sonicated ARSL, respectively). Arsonoliposomes may be composed only of arsonolipids (containing or not cholesterol) [plain ARSL], or they may contain mixtures of arsonolipids with phospholipids (with or without Chol) [mixed ARSL]. Herein, we describe in detail the preparation and physicochemical characterization of ARSL.