The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection
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The amino acids we have the most experience with are GABA, glutamate and arginine, though other amino acids can be measured. The method used is based on a derivatization reported by Jacobs (1 ) using o-phthalaldehyde in the presence of sulphite. The method was used by Pearson et al. (2 ), Smith and Sharp (3 ), and was further developed by Rowley et al. (4 ).
![(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-({[1-(tert-butoxy)-2-methyl-1-oxopropan-2-yl]oxy}imino)acetic acid，86299-47-0，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/707/8448654351228633081.jpg!wh200)
![DBD-COCl [=4-(N,N-二甲基氨磺酰)-7-(N-氯甲酰甲基-N-甲氨基)-2,1,3-苯并恶二唑][HPLC标记用]，156153-43-4，阿拉丁](https://img1.dxycdn.com/p/s14/2024/0619/381/2760431817860733081.jpg!wh200)
