Detection of Intracellular/lntranuclear Antigens: Applications in Leukemia/Lymphoma Analysis

The increased utilization of flow cytometry for the study of cell-associated antigens has paralleled the continuing development of new monoclonal antibodies (MAbs) specific for cell-associated antigens. Availability of these reagents, combined with the introduction of newer fluorochromes such as phycoerythrin (PE) and peridmin chlorophyll (PERCp) or tandem conjugates such as PE-Cy5 that may be used in conjunction with fluorescein isothiocyanate (FITC) allow simultaneous measurement of coexpression of three or four different antigens. Flow cytometry instrumentation also has evolved to the point that three- and four-color analysis is routinely available in simple benchtop instruments, not just on high powered sorting instruments.