Immunofluorescence of C. elegans Embryos
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Materials:
fixative |
dependent on antigen (common: 4% formaldehyde or 100% MeOH) |
post-fixative |
dependent on antigen (usually MeOH) |
PBS |
1X PBS |
PBST |
PBS + detergent (0.05 to 0.5 % Tween or Triton) |
DAPI (1000X) |
1 ug/ml in EtOH |
Antifade (BIORAD) |
|
Block |
1X PBS 1% BSA 10% Donkey Serum 0.02% Azide |