【共享】3i胶体金经典文献3－硝酸纤维素膜上蛋白质固定的问题解决

Troubleshooting protein binding in nitrocellulose membranes

(硝酸纤维素膜上蛋白质固定的问题解决)

发布者：派坤学苑 （bbs.3i.com.cn）

发布时间：2006-5-11

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Part 1: Principles

Kevin D. Jones

Developers of membrane-based assays should have a firm grasp on the various factors that can influence protein binding—including those inherent in the materials and processing used for their tests.

The number of membrane-based rapid immunochromatographic devices on the market is continuing to increase at a very quick pace. Major factors that are contributing to this growth include improvements in conjugate technology and a growing understanding among product developers of the general design principles involved.

市面上的基于膜快速免疫层析装置的数量以非常快的速度增长。这个增长的主要原因包括金标技术的提高和产品开发者之间了解的增多。

Although today's immunochromatographic devices come in a wide variety of designs with a diverse assortment of housings, most commercially available tests are based on one of two simple formats. The most common format is the lateral-flow or dipstick design, which has become familiar through its use in physician-office assays as well as in over-the-counter tests (e.g., Unipath's Clear Blue pregnancy test). A less widespread format is the flow-through or transverse-flow design, which requires greater operator skill and is therefore usually restricted to professional use (e.g., Medmira's rapid HIV screen).　

虽然今天的免疫层析装置在不同的分类中有很多种产品设计，上市最多的试剂是基于两种简单形式之一。最普通的形式是侧向流动或试纸条设计，通过在医生诊所检测和直接销售给用户的方式，使得它很常见。另一种较少见的形式是渗透设计，需要较强的操作技巧，因此只限于专业使用。