【资源】ambion已公布有效的siran序列
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Published siRNA Target Sequences
April 2003 - The significant increase in the rate of publication of siRNA target sequences has made it difficult to maintain a comprehensive list of these sequences. Therefore, we will no longer be updating this list. We apologize for any inconvenience this may cause.
The following siRNA target sequences have been used in published gene silencing experiments. The extent of silencing, which was assessed by various methods including immunodetection, Western blotting, and phenotypic analysis, varied among the targets. Please see the indicated references for experimental details.
Gene (Ref)
Accession #
Position from ATG
Gene Target
(Comments)
Cell Line Tested
siRNA Sequenceundefined
Template Sequenceundefined~Kbr_~H~M~2~1~Kbr_~H~M~2~1ß-actin (1)
NM_001101
972-994
aatgaagatcaagatcattgc
Human HeLa
View, Order
View
gamma-actin (1)
NM_001614
8-30
aagagatcgccgcgctggtca
Human HeLa
View, Order
View
AIB1 (estrogen receptor coactivator) (22) AAAGACUCCUUAGGACCGCUU
Ishikawa
View, Order
View
Apaf-1 (31)
978-998
AATTGGTGCACTTTTACGTGA
IMR90E1
View, Order
View
ARC21 (1)
AF006086
181-203
aagaatgaagctgataggacc
Human HeLa
View, Order
View
ATR interacting protein (4)
AF451323
254-274
AAGGTCCACAGATTATTAGAT
Human HeLa
View, Order
View
ATR interacting protein (4)
AF451323
464-484
CTAGAGGAACAGAGAAGATCACA
Human HeLa
View, Order
ATR interacting protein (4)
AF451323
867-887
AAGAAGAGGCCCAGAAAAGCT
Human HeLa
View, Order
View
Bcr-abl (32)
AAGCAGAGUUCAAAAGCCCUU
TonB, K562, hematopoetic cells
View, Order
View
Brc-abl (32)
AAAGCAGAGUUCAAAAGCCCU
TonB, K562, hematopoetic cells
View, Order
View
beta-tubulin- mouse neuronal (21) AF312873
AAGACAGAGCCAAGUGGACUC
Mouse P19
View, Order
View
Bruton's tyrosine kinase (Btk) (33) NM_000061
1682-1700
AAUUGGUAAACGAUCAAGGAG
RBL-2H3, WEHI-231, NALM-6
View, Order
View
Bruton's tyrosine kinase (Btk) (33) NM_000061
895-913
AAGGGAAAGAAGGAGGUUUCA
RBL-2H3, WEHI-231, NALM-6
View, Order
View
Bruton's tyrosine kinase (Btk) (33) NM_000061
518-536
AAGAAGCUUAAAACCUGGGAG
RBL-2H3, WEHI-231, NALM-6
View, Order
View
Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17)
520-538
AACUGGGGAAGAAGGGCAACC
HEK293
View, Order
View
Caspase 1 (34)
191-211
AAGGGGCACAGGCATGCCAAA
IMR90E1A
View, Order
View
Caspase 2 (34)
94-114
AAACAGCTGTTGTTGAGCGAA
IMR90E1A
View, Order
View
hCdc2 (24)
125-147
HeLa
Cdc14A (23)
89-109
GCACAGTAAATACCCACTATT
HeLa
View, Order
View
cdk1 (1)
NM_001786
125-147
aaggggttcctagtactgcaa
Human HeLa
View, Order
View
CENP-E (1)
NM_001813
944-966
aaactcttactgctctccagt
Human HeLa
View, Order
View
CHO1 (20)
138-156
AAGGUCAGUAAUACAACGGUG
CHO
View, Order
View
corin, mouse (12)
AACCACCGCCCUCCGUGCA
HL-5 and 293
Cyclin B1 (5)
J03166
199-217
CTCGTACAGCCTTGGGAGACA
Xenopus embryo
View, Order
Cyclin B2 (5)
J03167
252-270
ATGTTAAGACTAAGCATGTAC
Xenopus embryo
View, Order
cytoplasmic dynein 1 heavy chain (1)
U53530
509-531
aaggccaaggaggcgctggaa
Human HeLa
View, Order
View
DIP13-alpha (DCC interacting protein) (25) AAGUGCAGCAACACACCUGAC
DLD1
View, Order
View
DIP13-beta (DCC interacting protein) (25) AAGCAGCACCUCUCCUCCCUU
DLD1
View, Order
View
Dishevelled (hDv-1 and hDv-3) (16)
AACAAGATCACCTTCTCCGAG
A293
View, Order
View
hDv-2 (16)
AACTTTGAGAACATGAGCAAC
A293
View, Order
View
Eg5 (1)
NM_004523
1547-1569
aactggatcgtaagaaggcag
Human HeLa
View, Order
View
emerin (1)
NM_000117
628-650
aaccgtgctcctggggctggg
Human HeLa
View, Order
View
eve (11)
AACUCCUUGAACGGCAGCCGC
Drosophila embryos
View, Order
View
hFbw7 (cyclin E ubiquitination) (26)
713-735
see reference
HeLa
Fortilin (35)
121-141
AAGGUAACAUUGAUGACUCGC
U2OS
View, Order
View
GalTII (galactosyltransferase II) (27)
65-87
AAGCACGCGACGCUGGCGCGC
HeLa
View, Order
View
GalTII (galactosyltransferase II) (27)
461-482
AAGGCGGACGACGACUCCUUC
HeLa
View, Order
View
GAS41 (1)
NM_006530
327-349
aagacctgtaaccctgtatca
Human HeLa
View, Order
View
Hec-1 (36) NM_006101
1517-1539
HeLa-S3
HPV E6 (38)
AAGAGGUAUAUGACUUUGCUU
CaSKi, SiHa, NDF, HCT116
View, Order
View
HPV E7 (38)
AAAGGAGGAUGAAAUAGAUGG
CaSKi, SiHa, NDF, HCT116
View, Order
View
HtrA2 (28) AAAACGGCUCAGGAUUCGUGG
U2OS
View, Order
View
HtrA2 (28) AAGGGGAGTTTGTTGTTGCCA
U2OS
View, Order
View
HtrA2 (28) AAGCTTTCCCGATGTTCAGCA View, Order
View
keratin 18 (1)
NM_000224
1154-1176
aagatggcgaggactttaatc
Human HeLa
View, Order
View
Kinase interacting stathmin (hKIS) (39)
160-180
AAGCAGTTCTTGCCGCCAGGA
HEK293
View, Order
View
Kinase interacting stathmin (mKIS) (39)
160-180
AAGCAGTTCCTGCCTCGGGGA
HEK293
View, Order
View
lamin A (13)
NM019390
260-280
GAAGCAGCUUCAGGAUGAGAU
Human HeLa S3
lamin A (19)
AAGGACCUGGAGGUCUGCUG
Hep3B
lamin A/C (1)
NM_005572
608-630
aactggacttccagaagaaca
(90% reduction; 2)
Human HeLa
View, Order
View
lamin B1 (1)
NM_005573
672-694
aacgcgcttggtagaggtgga
Human HeLa
View, Order
View
lamin B2 (1)
M94362
1457-1479
aagaggaggaggaagccgagt
Human HeLa
View, Order
View
LAP2 (1)
NM_003276
37-59
aaagacaagttgaagagtgag
Human HeLa
View, Order
View
Mad1 (41)
AF083811
1843-1865
See reference
HeLa-S3
Mammalian septin (MSF) (44)
AF189712
472-492
See reference
HeLa
Methyl-CpG-binding protein 2 (MeCP2) (19)
AAGCAUGAGCCCGUGCAGCCA
Hep3B
View, Order
View
Methyl-CpG binding domain protein 2 (MBD2) (19)
AAGAGGAUGGAUUGCCCGGCC
Hep3B
View, Order
View
Microtubule-associated protein 2 (MAP2) (42)
AAUCCUUCGUCUUUCCUCUCG
primary neuronal cells
View, Order
View
Myc-induced nuclear antigen 53 (mina53) (40)
45-63
See reference
HeLa, MycB
MPS1 kinase (hMps1) (8)
133-155
See reference
Human HeLa S3
Myeloid cell leukemia protein 1 (MCL1) (43)
456-476
AACCCGUCCGUACUGGUGUUA
U2OS
View, Order
View
Nicastrin (Nct) (45)
AAGGGCAAGTTTCCCGTGCAG
HEK293
View, Order
View
NuMA (1)
Z11583
3988-4010
aaggcgtggcaggagaagttc
Human HeLa
View, Order
View
Nup153 (1)
NM_005124
2593-2615
aaggcagactctaccaaatgt
Human HeLa
View, Order
View
Origin recognition complex protein 6 (Orc6) (46)
AAUGGUAGCCACAUCCGGUGU
HeLa
View, Order
View
Origin recognition complex protein 6 (Orc6) (46)
AAGATTGGACAGCAGGUCGAC
HeLa
View, Order
View
P160 ROCK (47)
566-584; 639-657; 1958-1976; 2780-2798
See reference
Polo-like Kinase1 (hPlk1) (37) NM_005030
181-203
AAGGGCGGCTTTGCCAAGTGC
HeLa, U2OS
View, Order
View
Protein Kinase Receptor (PKR) (9)
931-949
AAGATCAAGTTTTGCCAATG
Human HeLa
hRad9 (29)
U53174
232-252
AAGUCUUUCCUGUCUGUCUUU
MCF-7
View, Order
View
Rad17 (7)
AACAGACUGGGUUGACCCAUC
Human HeLa
View, Order
View
Signalling Adapter/scaffold protein (h/m Shc) (3)
HSU73377
677-697
tcctacttggttcggtacatggg
Human HeLa
View, Order
p66 ShcA (3)
HSU73377
236-256
CGGAATGAGTCTCTGTCATCGTC
Human HeLa
View, Order
Sphingosine kinase 1 (hSK1) (15)
70 nt downstream of start codon
AAGAGCUGCAAGGCCUUGCCC
HUVEC
View, Order
View
Steroid receptor coactivator (SRC-1) (30) CCUCAGGGCAGAGAACCAUCUTT
Ishikawa
ß-tubulin (6)
AF312873
3'-UTR
GACAGAGCCAAGUGGACUCAC
Mouse P19
Tissue Factor (hTF) (18)
M16553
AAGCGCUUCAGGCACUACAAA
HeLa, 293, Cos-1, HaCaT
View, Order
View
Tissue Factor (hTF) (18)
M16553
AAGAAGCAGACGUACUUGGCA
HeLa, 293, Cos-1, HaCaT
View, Order
View
ß-transducin repeat-containing protein (ß-TrCP), human (10)
GUGGAAUUUGUGGAACAUCTT
293
View, Order
View
TSG101 (a ubiquitin enzyme 2 variant protein) (14)
413-433
AACCUCCAGUCUUCUCUCGUC
Human HeLa-M & HeLa KK3
View, Order
View
VASP (1)
XM_009141
1087-1109
aaagaggaaatcattgaagcc
Human HeLa
View, Order
View
vimentin (1)
NM_003380
346-368
aactacatcgacaaggtgcgc
(No silencing observed; 1,2)
Human HeLa
View, Order
View
vimentin (1)
NM_003380
1145-1167
aataccaagacctgctcaatg
Human HeLa
View, Order
View
vimentin (1)
NM_003380
863-885
aagaatggtacaaatccaagt
Human HeLa
View, Order
View
vimentin (1)
NM_003380
1037-1059
aaatggaagagaactttgccg
Human HeLa
View, Order
View
vinculin, mouse (1)
L18880
2923-2945
aagcggattagaaccaatctc
Mouse 3T3
View, Order
View
zyxin, mouse (1)
X99063
1357-1379
aaagcctaccacccacagtgc
Mouse 3T3
View, Order
View
~undefined"View" shows the sense and antisense strands of the siRNA corresponding to the Gene Target. "Order" leads to a custom siRNA order form with the appropriate siRNA sequence pre-filled out. See Ambion's Custom siRNA Oligonucleotide Synthesis Service for details.
~undefinedTemplate_sequences_for_the_Silencer™ siRNA Construction Kit.
References
1. Harborth J, Elbashir SM, Bechert K, Tuschl T, and Weber K. (2001) Identification of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Science 114: 4557-4565.
2. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, and Tuschl T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494-498.
3. Kisielow M, Kleiner S, Nagasawa M, Faisal A, and Nagamine Y. (2002) Isoform-specific knockdown and expression of adapter protein ShcA using small interfering RNA. Biochem. J. 363:1-5.
4. Cortez D, Guntuku S, Qin J, and Elledge SJ. (2001) ATR and ATRIP: partners in checkpoint signaling. Science 294:1713-1716.
5. Zhou Y, Ching Y-P, Kok KH, Kung H-F, Jin D-Y. (2002). Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA. Nucleic Acids Res 30(7):1664-1669.
6. Yu J-Y, DeRuiter SL, Turner DL. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99(9):6047-6052.
7. Zou L, Cortez D, and Elledge SJ. (2002). Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin. Genes & Devel. 16:198-208.
8. Stucke VM, Sillje HHW, Arnaud L, and Nigg EA. (2002). Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication. EMBO 21(7):1723-1732.
9. Donze O, and Picard D. (2002). RNA interference in mammalian cells using siRNAs synthesized with T7 polymerase. Nucl. Acids Res. 30(10): e46.
10. Fong A, and Sun S-C. (May 6, 2002). Genetic evidence for the essential role of beta-transducin repeat-containing protein in the inducible processing of NF-kB2/p100. J. Biol. Chem. (preprint).
11. Williams R, and Rubin GM. (2002). ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proc. Natl. Acad. Sci. USA 99(10):6889-6894.
12. Wu F, Yan W, Pan J, Morser J, and Wu Q. (2002). Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes. J. Biol. Chem. 277(19):16900-16905.
13. Bai, X, Zhou, D, Brown, JR, Crawford, BE, Hennet, T, and Esko, JD. (2001). Biosynthesis of the linkage region of glycosaminoglycans. J. Biol. Chem. 276(51):48189-48195.
14. Hewitt EW, Duncan L, Mufti D, Baker J, Stevenson PG, and Lehner PJ. (2002). Ubiquitylation of MHC class I by the K3 viral protein signals internalization and TSG101-dependent degradation. EMBO 21(10): 2418-2429.
15. Ancellin, N, Colmont, C, Su, J, Li, Q, Mittereder, N, Chae, S-S, Stefansson, S, Liau, G, and Hla, T. (2002). Extracelllular export of sphingosine kinase-1 enzyme. J. Biol. Chem. 277(8):6667-6675.
16. Li L, Mao J, Sun L, Liu W, and Wu D. (2002). Second cysteine-rich domain of Dickkopf-2 activates canonical Wnt signaling pathway via LRP-6 independently of Dishevelled. J. Biol. Chem. 277(8):5977-5981.
17. Eide EJ, Bielhaber EL, Hinz W, and Virshup DM. (2002). The circadian regulatory proteins BMAL1 and Cryptochromes are substrates of casein kinase I epsilon. J. Biol. Chem. 277(19):17248-17254.
18. Holen T, Amarzguioui M, Wiige, MT, Babaie E, and Prydz H. (2002). Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor. Nucl. Acids Res. 30(8):1757-1766.
19. Bakker J, Lin X, and Nelson WG. (2002). Methyl-CpG binding domain protein 2 represses transcription from hypermethylated p-class glutathione-S-transferase gene promoters in hepatocellular carcinoma cells. J. Biol. Chem. (in press).
20. Matuliene J, and Kuriyama R. (17 April 2002). Kinesin-like protein CHO1 is required for the formation of midbody matrix and the completion of cytokinesis in mammalian cells. Mol. Biol. Cell. (in press).
21. Yu, J-Y, DeRuiter, SL, Turner, DL. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99(9):6047-6052.
22. Shang Y and Brown M. (2002). Molecular determinants for the tissue specificity of SERMs. Science 295:2465-2468.
23. Mailand N, Lukas C, Kaiser BK, Jackson PK, Bartek J, and Lukas J. (2002). Deregulated human Cdc14A phosphatase disrupts centrosome separation and chromosome segregation. Nature Cell Biol. 4:317-322.
24. Chang T-S, Jeong W, Choi SY, Yu S, Kang SW, and Rhee SG. (2002). Regulation of peroxiredoxin I activity by Cdc2-mediated phosphorylation. J. Biol. Chem. 277(28):25370-25376.
25. Liu J, Yao F, Wu R, Morgan M, Thorburn A, Finely RL, Chen YQ. (2002). Mediation of the DCC apoptotic signal by DIP13 alpha. J. Biol. Chem. 277(29):26281-26285.
26. Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW, and Elledge SJ. (2001). Phosphorylation-dependent ubiquitination of cyclin E by the SCF-Fbw7 ubiquitin ligase. Science 294(5540):173-177.
27. Bai, X, Zhou, D, Brown, JR, Crawford, BE, Hennet, T, and Esko, JD. (2001). Biosynthesis of the linkage region of glycosaminoglycans. J. Biol. Chem. 276(51):48189-48195.
28. Martins et al. (2002). The serine protease Omi/HtrA2 regulates apoptosis by binding XIAP through a reaper like motif. J. Biol. Chem. 277:439-444.
29. Hirai H and Wang H-G. (2002). A role of the C-terminal region of human Rad9 (hRad9) in nuclear transport of the hRad9 checkpoint complex. J. Biol. Chem. 277(28):25722-25727.
30. Shang Y and Brown M. (2002). Molecular determinants for the tissue specificity of SERMs. Science 295:2465-2468.
31. Lassus P, Opitz-Araya X, and Lazebnik Y. (2002). Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 297:1352-1354.
32. Scherr M, Battmer K, Winkler T, Heidenreich O, Ganser A, and Eder M. (2002). Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood (online September 26, 2002 issue)
33. Heinonen JE, Smith CIE, and Nore BF. (2002). Silencing of Bruton's tyrosine kinase (Btk) using short interfering RNA duplexes (siRNA). FEBS Lett 527:274-278.
34. Lassus P, Opitz-Araya X, and Lazebnik Y. (2002). Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 297:1352-1354.
35. Zhang D, Li F, Weidner D, Mnjoyan ZH, and Fujise K. (2002). Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and fortilin. J. Biol. Chem. 277(40):37430-37438.
36. Martin-Lluesma S, Stucke VM, and Nigg EA. (2002). Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 297:2267-2270.
37. Liu X and Erikson RL. (2002). Activation of Cdc2/cyclin B and inhibition of centromere amplification in cells depleted of Plk1 by siRNA. Proc. Natl. Acad. Sci. USA 99(13):8672-8676.
38. Jiang M and Milner J. (2002). Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene 21:6041-6048.
39. Boehm M, Yoshimoto T, Crook MF, Nallamshetty S, True A, Nabel G, and Nabel EG. (2002). A growth factor-dependent nuclear kinase phosphorylates p27kip1 and regulates cell cycle progression. EMBO J. 21(13):3390-3401.
40. Tsuneoka M, Koda Y, Soejima M, Teye K, and Kimura H. (2002). A novel Myc target gene, mina53, that is involved in cell proliferation. J. Biol. Chem. 277(38):35450-35459.
41. Martin-Lluesma S, Stucke VM, and Nigg EA. (2002). Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 297:2267-2270.
42. Krichevsky AM and Kosik KS. (2002). RNAi functions in cultured mammalian neurons. Proc. Natl. Acad. Sci USA 99(18):11926-11929.
43. Zhang D, Li F, Weidner D, Mnjoyan ZH, and Fujise K. (2002). Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and fortilin. J. Biol. Chem. 277(40):37430-37438.
44. Surka MC, Tsang CW, and Trimble WS. (2002). The mammalian septin MSF localizes with microtubules and is required for completion of cytokinesis. Mol Biol. Cell 13:3532-3545.
45. Edbauer D, Winkler E, Haass C, and Steiner H. (2002). Presenilin and nicastrin regulate each other and determine amyloid b-peptide production via complex formation. Proc. Natl. Acad. Sci. USA 99(13):8666-8671.
46. Prasanth SG, Prasanth KV, and Stillman B. (2002). Orc6 involved in DNA replication, chromosome segregation, and cytokinesis. Science 297:1026-1031.
47. Chevrier V, Piel M, Collomb N, Saoudi Y, Frank R, Paintrand M, Narumiya S, Bornens M, and Job D. (2002). The Rho-associated protein kinase p160ROCK is required for centrosome positioning. J. Cell Biol. 157:807-817.
Published siRNA Target Sequences
April 2003 - The significant increase in the rate of publication of siRNA target sequences has made it difficult to maintain a comprehensive list of these sequences. Therefore, we will no longer be updating this list. We apologize for any inconvenience this may cause.
The following siRNA target sequences have been used in published gene silencing experiments. The extent of silencing, which was assessed by various methods including immunodetection, Western blotting, and phenotypic analysis, varied among the targets. Please see the indicated references for experimental details.
Gene (Ref)
Accession #
Position from ATG
Gene Target
(Comments)
Cell Line Tested
siRNA Sequenceundefined
Template Sequenceundefined~Kbr_~H~M~2~1~Kbr_~H~M~2~1ß-actin (1)
NM_001101
972-994
aatgaagatcaagatcattgc
Human HeLa
View, Order
View
gamma-actin (1)
NM_001614
8-30
aagagatcgccgcgctggtca
Human HeLa
View, Order
View
AIB1 (estrogen receptor coactivator) (22) AAAGACUCCUUAGGACCGCUU
Ishikawa
View, Order
View
Apaf-1 (31)
978-998
AATTGGTGCACTTTTACGTGA
IMR90E1
View, Order
View
ARC21 (1)
AF006086
181-203
aagaatgaagctgataggacc
Human HeLa
View, Order
View
ATR interacting protein (4)
AF451323
254-274
AAGGTCCACAGATTATTAGAT
Human HeLa
View, Order
View
ATR interacting protein (4)
AF451323
464-484
CTAGAGGAACAGAGAAGATCACA
Human HeLa
View, Order
ATR interacting protein (4)
AF451323
867-887
AAGAAGAGGCCCAGAAAAGCT
Human HeLa
View, Order
View
Bcr-abl (32)
AAGCAGAGUUCAAAAGCCCUU
TonB, K562, hematopoetic cells
View, Order
View
Brc-abl (32)
AAAGCAGAGUUCAAAAGCCCU
TonB, K562, hematopoetic cells
View, Order
View
beta-tubulin- mouse neuronal (21) AF312873
AAGACAGAGCCAAGUGGACUC
Mouse P19
View, Order
View
Bruton's tyrosine kinase (Btk) (33) NM_000061
1682-1700
AAUUGGUAAACGAUCAAGGAG
RBL-2H3, WEHI-231, NALM-6
View, Order
View
Bruton's tyrosine kinase (Btk) (33) NM_000061
895-913
AAGGGAAAGAAGGAGGUUUCA
RBL-2H3, WEHI-231, NALM-6
View, Order
View
Bruton's tyrosine kinase (Btk) (33) NM_000061
518-536
AAGAAGCUUAAAACCUGGGAG
RBL-2H3, WEHI-231, NALM-6
View, Order
View
Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17)
520-538
AACUGGGGAAGAAGGGCAACC
HEK293
View, Order
View
Caspase 1 (34)
191-211
AAGGGGCACAGGCATGCCAAA
IMR90E1A
View, Order
View
Caspase 2 (34)
94-114
AAACAGCTGTTGTTGAGCGAA
IMR90E1A
View, Order
View
hCdc2 (24)
125-147
HeLa
Cdc14A (23)
89-109
GCACAGTAAATACCCACTATT
HeLa
View, Order
View
cdk1 (1)
NM_001786
125-147
aaggggttcctagtactgcaa
Human HeLa
View, Order
View
CENP-E (1)
NM_001813
944-966
aaactcttactgctctccagt
Human HeLa
View, Order
View
CHO1 (20)
138-156
AAGGUCAGUAAUACAACGGUG
CHO
View, Order
View
corin, mouse (12)
AACCACCGCCCUCCGUGCA
HL-5 and 293
Cyclin B1 (5)
J03166
199-217
CTCGTACAGCCTTGGGAGACA
Xenopus embryo
View, Order
Cyclin B2 (5)
J03167
252-270
ATGTTAAGACTAAGCATGTAC
Xenopus embryo
View, Order
cytoplasmic dynein 1 heavy chain (1)
U53530
509-531
aaggccaaggaggcgctggaa
Human HeLa
View, Order
View
DIP13-alpha (DCC interacting protein) (25) AAGUGCAGCAACACACCUGAC
DLD1
View, Order
View
DIP13-beta (DCC interacting protein) (25) AAGCAGCACCUCUCCUCCCUU
DLD1
View, Order
View
Dishevelled (hDv-1 and hDv-3) (16)
AACAAGATCACCTTCTCCGAG
A293
View, Order
View
hDv-2 (16)
AACTTTGAGAACATGAGCAAC
A293
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View
Eg5 (1)
NM_004523
1547-1569
aactggatcgtaagaaggcag
Human HeLa
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View
emerin (1)
NM_000117
628-650
aaccgtgctcctggggctggg
Human HeLa
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View
eve (11)
AACUCCUUGAACGGCAGCCGC
Drosophila embryos
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View
hFbw7 (cyclin E ubiquitination) (26)
713-735
see reference
HeLa
Fortilin (35)
121-141
AAGGUAACAUUGAUGACUCGC
U2OS
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View
GalTII (galactosyltransferase II) (27)
65-87
AAGCACGCGACGCUGGCGCGC
HeLa
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View
GalTII (galactosyltransferase II) (27)
461-482
AAGGCGGACGACGACUCCUUC
HeLa
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GAS41 (1)
NM_006530
327-349
aagacctgtaaccctgtatca
Human HeLa
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View
Hec-1 (36) NM_006101
1517-1539
HeLa-S3
HPV E6 (38)
AAGAGGUAUAUGACUUUGCUU
CaSKi, SiHa, NDF, HCT116
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View
HPV E7 (38)
AAAGGAGGAUGAAAUAGAUGG
CaSKi, SiHa, NDF, HCT116
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HtrA2 (28) AAAACGGCUCAGGAUUCGUGG
U2OS
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View
HtrA2 (28) AAGGGGAGTTTGTTGTTGCCA
U2OS
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View
HtrA2 (28) AAGCTTTCCCGATGTTCAGCA View, Order
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keratin 18 (1)
NM_000224
1154-1176
aagatggcgaggactttaatc
Human HeLa
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View
Kinase interacting stathmin (hKIS) (39)
160-180
AAGCAGTTCTTGCCGCCAGGA
HEK293
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View
Kinase interacting stathmin (mKIS) (39)
160-180
AAGCAGTTCCTGCCTCGGGGA
HEK293
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lamin A (13)
NM019390
260-280
GAAGCAGCUUCAGGAUGAGAU
Human HeLa S3
lamin A (19)
AAGGACCUGGAGGUCUGCUG
Hep3B
lamin A/C (1)
NM_005572
608-630
aactggacttccagaagaaca
(90% reduction; 2)
Human HeLa
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lamin B1 (1)
NM_005573
672-694
aacgcgcttggtagaggtgga
Human HeLa
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lamin B2 (1)
M94362
1457-1479
aagaggaggaggaagccgagt
Human HeLa
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LAP2 (1)
NM_003276
37-59
aaagacaagttgaagagtgag
Human HeLa
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Mad1 (41)
AF083811
1843-1865
See reference
HeLa-S3
Mammalian septin (MSF) (44)
AF189712
472-492
See reference
HeLa
Methyl-CpG-binding protein 2 (MeCP2) (19)
AAGCAUGAGCCCGUGCAGCCA
Hep3B
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View
Methyl-CpG binding domain protein 2 (MBD2) (19)
AAGAGGAUGGAUUGCCCGGCC
Hep3B
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Microtubule-associated protein 2 (MAP2) (42)
AAUCCUUCGUCUUUCCUCUCG
primary neuronal cells
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Myc-induced nuclear antigen 53 (mina53) (40)
45-63
See reference
HeLa, MycB
MPS1 kinase (hMps1) (8)
133-155
See reference
Human HeLa S3
Myeloid cell leukemia protein 1 (MCL1) (43)
456-476
AACCCGUCCGUACUGGUGUUA
U2OS
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View
Nicastrin (Nct) (45)
AAGGGCAAGTTTCCCGTGCAG
HEK293
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NuMA (1)
Z11583
3988-4010
aaggcgtggcaggagaagttc
Human HeLa
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Nup153 (1)
NM_005124
2593-2615
aaggcagactctaccaaatgt
Human HeLa
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Origin recognition complex protein 6 (Orc6) (46)
AAUGGUAGCCACAUCCGGUGU
HeLa
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View
Origin recognition complex protein 6 (Orc6) (46)
AAGATTGGACAGCAGGUCGAC
HeLa
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P160 ROCK (47)
566-584; 639-657; 1958-1976; 2780-2798
See reference
Polo-like Kinase1 (hPlk1) (37) NM_005030
181-203
AAGGGCGGCTTTGCCAAGTGC
HeLa, U2OS
View, Order
View
Protein Kinase Receptor (PKR) (9)
931-949
AAGATCAAGTTTTGCCAATG
Human HeLa
hRad9 (29)
U53174
232-252
AAGUCUUUCCUGUCUGUCUUU
MCF-7
View, Order
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Rad17 (7)
AACAGACUGGGUUGACCCAUC
Human HeLa
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View
Signalling Adapter/scaffold protein (h/m Shc) (3)
HSU73377
677-697
tcctacttggttcggtacatggg
Human HeLa
View, Order
p66 ShcA (3)
HSU73377
236-256
CGGAATGAGTCTCTGTCATCGTC
Human HeLa
View, Order
Sphingosine kinase 1 (hSK1) (15)
70 nt downstream of start codon
AAGAGCUGCAAGGCCUUGCCC
HUVEC
View, Order
View
Steroid receptor coactivator (SRC-1) (30) CCUCAGGGCAGAGAACCAUCUTT
Ishikawa
ß-tubulin (6)
AF312873
3'-UTR
GACAGAGCCAAGUGGACUCAC
Mouse P19
Tissue Factor (hTF) (18)
M16553
AAGCGCUUCAGGCACUACAAA
HeLa, 293, Cos-1, HaCaT
View, Order
View
Tissue Factor (hTF) (18)
M16553
AAGAAGCAGACGUACUUGGCA
HeLa, 293, Cos-1, HaCaT
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View
ß-transducin repeat-containing protein (ß-TrCP), human (10)
GUGGAAUUUGUGGAACAUCTT
293
View, Order
View
TSG101 (a ubiquitin enzyme 2 variant protein) (14)
413-433
AACCUCCAGUCUUCUCUCGUC
Human HeLa-M & HeLa KK3
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View
VASP (1)
XM_009141
1087-1109
aaagaggaaatcattgaagcc
Human HeLa
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View
vimentin (1)
NM_003380
346-368
aactacatcgacaaggtgcgc
(No silencing observed; 1,2)
Human HeLa
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View
vimentin (1)
NM_003380
1145-1167
aataccaagacctgctcaatg
Human HeLa
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View
vimentin (1)
NM_003380
863-885
aagaatggtacaaatccaagt
Human HeLa
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View
vimentin (1)
NM_003380
1037-1059
aaatggaagagaactttgccg
Human HeLa
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View
vinculin, mouse (1)
L18880
2923-2945
aagcggattagaaccaatctc
Mouse 3T3
View, Order
View
zyxin, mouse (1)
X99063
1357-1379
aaagcctaccacccacagtgc
Mouse 3T3
View, Order
View
~undefined"View" shows the sense and antisense strands of the siRNA corresponding to the Gene Target. "Order" leads to a custom siRNA order form with the appropriate siRNA sequence pre-filled out. See Ambion's Custom siRNA Oligonucleotide Synthesis Service for details.
~undefinedTemplate_sequences_for_the_Silencer™ siRNA Construction Kit.
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