【资源】ambion已公布有效的siran序列

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Published siRNA Target Sequences

April 2003 - The significant increase in the rate of publication of siRNA target sequences has made it difficult to maintain a comprehensive list of these sequences. Therefore, we will no longer be updating this list. We apologize for any inconvenience this may cause.

The following siRNA target sequences have been used in published gene silencing experiments. The extent of silencing, which was assessed by various methods including immunodetection, Western blotting, and phenotypic analysis, varied among the targets. Please see the indicated references for experimental details.

Gene (Ref)
  

Accession #
  

Position from ATG
  

Gene Target
(Comments)
  

Cell Line Tested
  

siRNA Sequenceundefined
  

Template Sequenceundefined~Kbr_~H~M~2~1~Kbr_~H~M~2~1ß-actin (1)
  

NM_001101
  

972-994
  

aatgaagatcaagatcattgc
  
Human HeLa
  
View, Order
  
View

gamma-actin (1)
  

NM_001614
  

8-30
  

aagagatcgccgcgctggtca
  
Human HeLa
  
View, Order
  
View
AIB1 (estrogen receptor coactivator) (22)         AAAGACUCCUUAGGACCGCUU   
Ishikawa
  View, Order   
View
Apaf-1 (31)      
978-998
  
AATTGGTGCACTTTTACGTGA
  
IMR90E1
  View, Order   
View

ARC21 (1)
  

AF006086
  

181-203
  

aagaatgaagctgataggacc
  
Human HeLa
  
View, Order
  
View
ATR interacting protein (4)   
AF451323
  
254-274
  
AAGGTCCACAGATTATTAGAT
  
Human HeLa
  
View, Order
  
View
ATR interacting protein (4)   
AF451323
  
464-484
  
CTAGAGGAACAGAGAAGATCACA
  
Human HeLa
  
View, Order
  

ATR interacting protein (4)   
AF451323
  
867-887
  
AAGAAGAGGCCCAGAAAAGCT
  
Human HeLa
  
View, Order
  
View
Bcr-abl (32)         
AAGCAGAGUUCAAAAGCCCUU
  TonB, K562, hematopoetic cells   
View, Order
  
View
Brc-abl (32)         
AAAGCAGAGUUCAAAAGCCCU
  TonB, K562, hematopoetic cells   
View, Order
  
View
beta-tubulin- mouse neuronal (21)   AF312873      
AAGACAGAGCCAAGUGGACUC
  
Mouse P19
  View, Order   
View
Bruton's tyrosine kinase (Btk) (33)   NM_000061   
1682-1700
  
AAUUGGUAAACGAUCAAGGAG
  
RBL-2H3, WEHI-231, NALM-6
  View, Order   
View
Bruton's tyrosine kinase (Btk) (33)   NM_000061   
895-913
  
AAGGGAAAGAAGGAGGUUUCA
  
RBL-2H3, WEHI-231, NALM-6
  View, Order   
View
Bruton's tyrosine kinase (Btk) (33)   NM_000061   
518-536
  
AAGAAGCUUAAAACCUGGGAG
  
RBL-2H3, WEHI-231, NALM-6
  View, Order   
View
Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17)   

  
520-538
  
AACUGGGGAAGAAGGGCAACC
  
HEK293
  
View, Order
  
View
Caspase 1 (34)      
191-211
  
AAGGGGCACAGGCATGCCAAA
  
IMR90E1A
  
View, Order
  
View
Caspase 2 (34)      
94-114
  
AAACAGCTGTTGTTGAGCGAA
  
IMR90E1A
  
View, Order
  
View
hCdc2 (24)      
125-147
     
HeLa
     
Cdc14A (23)      
89-109
  
GCACAGTAAATACCCACTATT
  
HeLa
  View, Order   
View

cdk1 (1)
  

NM_001786
  

125-147
  

aaggggttcctagtactgcaa
  
Human HeLa
  
View, Order
  
View

CENP-E (1)
  

NM_001813
  

944-966
  

aaactcttactgctctccagt
  
Human HeLa
  
View, Order
  
View
CHO1 (20)   

  
138-156
  
AAGGUCAGUAAUACAACGGUG
  
CHO
  
View, Order
  
View
corin, mouse (12)   

  

  
AACCACCGCCCUCCGUGCA
  
HL-5 and 293
     
Cyclin B1 (5)   
J03166
  
199-217
  
CTCGTACAGCCTTGGGAGACA
  
Xenopus embryo
  
View, Order
  

Cyclin B2 (5)   
J03167
  
252-270
  
ATGTTAAGACTAAGCATGTAC
  
Xenopus embryo
  
View, Order
  


cytoplasmic dynein 1 heavy chain (1)
  

U53530
  

509-531
  

aaggccaaggaggcgctggaa
  
Human HeLa
  
View, Order
  
View
DIP13-alpha (DCC interacting protein) (25)         AAGUGCAGCAACACACCUGAC   
DLD1
  View, Order   
View
DIP13-beta (DCC interacting protein) (25)         AAGCAGCACCUCUCCUCCCUU   
DLD1
  View, Order   
View
Dishevelled (hDv-1 and hDv-3) (16)
  

  

  
AACAAGATCACCTTCTCCGAG
  
A293
  
View, Order
  
View
hDv-2 (16)
  

  

  
AACTTTGAGAACATGAGCAAC
  
A293
  
View, Order
  
View

Eg5 (1)
  

NM_004523
  

1547-1569
  

aactggatcgtaagaaggcag
  
Human HeLa
  
View, Order
  
View

emerin (1)
  

NM_000117
  

628-650
  

aaccgtgctcctggggctggg
  
Human HeLa
  
View, Order
  
View
eve (11)   

  

  
AACUCCUUGAACGGCAGCCGC
  
Drosophila embryos
  
View, Order
  
View
hFbw7 (cyclin E ubiquitination) (26)      
713-735
  
see reference
  
HeLa
     
Fortilin (35)      
121-141
  
AAGGUAACAUUGAUGACUCGC
  
U2OS
  
View, Order
  
View
GalTII (galactosyltransferase II) (27)      
65-87
  AAGCACGCGACGCUGGCGCGC   
HeLa
  View, Order   
View
GalTII (galactosyltransferase II) (27)   
  
461-482
  AAGGCGGACGACGACUCCUUC   
HeLa
  View, Order   
View

GAS41 (1)
  

NM_006530
  

327-349
  

aagacctgtaaccctgtatca
  
Human HeLa
  
View, Order
  
View
Hec-1 (36)   NM_006101   
1517-1539
     
HeLa-S3
     
HPV E6 (38)         
AAGAGGUAUAUGACUUUGCUU
  
CaSKi, SiHa, NDF, HCT116
  
View, Order
  
View
HPV E7 (38)         
AAAGGAGGAUGAAAUAGAUGG
  CaSKi, SiHa, NDF, HCT116   
View, Order
  
View
HtrA2 (28)         AAAACGGCUCAGGAUUCGUGG   
U2OS
  View, Order   
View
HtrA2 (28)         AAGGGGAGTTTGTTGTTGCCA   
U2OS
  View, Order   
View
HtrA2 (28)         AAGCTTTCCCGATGTTCAGCA      View, Order   
View

keratin 18 (1)
  

NM_000224
  

1154-1176
  

aagatggcgaggactttaatc
  
Human HeLa
  
View, Order
  
View
Kinase interacting stathmin (hKIS) (39)      
160-180
  
AAGCAGTTCTTGCCGCCAGGA
  
HEK293
  
View, Order
  
View
Kinase interacting stathmin (mKIS) (39)      
160-180
  
AAGCAGTTCCTGCCTCGGGGA
  
HEK293
  
View, Order
  
View
lamin A (13)   
NM019390
  
260-280
  
GAAGCAGCUUCAGGAUGAGAU
  
Human HeLa S3
  

  

lamin A (19)   

  

  
AAGGACCUGGAGGUCUGCUG
  
Hep3B
  

  


lamin A/C (1)
  

NM_005572
  

608-630
  

aactggacttccagaagaaca
(90% reduction; 2)
  
Human HeLa
  
View, Order
  
View

lamin B1 (1)
  

NM_005573
  

672-694
  

aacgcgcttggtagaggtgga
  
Human HeLa
  
View, Order
  
View

lamin B2 (1)
  

M94362
  

1457-1479
  

aagaggaggaggaagccgagt
  
Human HeLa
  
View, Order
  
View

LAP2 (1)
  

NM_003276
  

37-59
  

aaagacaagttgaagagtgag
  
Human HeLa
  
View, Order
  
View
Mad1 (41)   
AF083811
  
1843-1865
  
See reference
  
HeLa-S3
     
Mammalian septin (MSF) (44)   
AF189712
  
472-492
  
See reference
  
HeLa
     
Methyl-CpG-binding protein 2 (MeCP2) (19)
  

  

  
AAGCAUGAGCCCGUGCAGCCA
  
Hep3B
  
View, Order
  
View
Methyl-CpG binding domain protein 2 (MBD2) (19)   

  

  
AAGAGGAUGGAUUGCCCGGCC
  
Hep3B
  
View, Order
  
View
Microtubule-associated protein 2 (MAP2) (42)         
AAUCCUUCGUCUUUCCUCUCG
  
primary neuronal cells
  
View, Order
  
View
Myc-induced nuclear antigen 53 (mina53) (40)      
45-63
  
See reference
  
HeLa, MycB
     
MPS1 kinase (hMps1) (8)   

  
133-155
  
See reference
  
Human HeLa S3
  

  

Myeloid cell leukemia protein 1 (MCL1) (43)      
456-476
  
AACCCGUCCGUACUGGUGUUA
  
U2OS
  
View, Order
  
View
Nicastrin (Nct) (45)         
AAGGGCAAGTTTCCCGTGCAG
  
HEK293
  
View, Order
  
View

NuMA (1)
  

Z11583
  

3988-4010
  

aaggcgtggcaggagaagttc
  
Human HeLa
  
View, Order
  
View

Nup153 (1)
  

NM_005124
  

2593-2615
  

aaggcagactctaccaaatgt
  
Human HeLa
  
View, Order
  
View
Origin recognition complex protein 6 (Orc6) (46)         
AAUGGUAGCCACAUCCGGUGU
  
HeLa
  
View, Order
  
View
Origin recognition complex protein 6 (Orc6) (46)         
AAGATTGGACAGCAGGUCGAC
  
HeLa
  
View, Order
  
View
P160 ROCK (47)      
566-584; 639-657; 1958-1976; 2780-2798
  
See reference
        
Polo-like Kinase1 (hPlk1) (37)   NM_005030   
181-203
  
AAGGGCGGCTTTGCCAAGTGC
  
HeLa, U2OS
  
View, Order
  
View
Protein Kinase Receptor (PKR) (9)   

  
931-949
  
AAGATCAAGTTTTGCCAATG
  
Human HeLa
  

  

hRad9 (29)   
U53174
  
232-252
  AAGUCUUUCCUGUCUGUCUUU   
MCF-7
  View, Order   
View
Rad17 (7)   

  

  
AACAGACUGGGUUGACCCAUC
  
Human HeLa
  
View, Order
  
View
Signalling Adapter/scaffold protein (h/m Shc) (3)   
HSU73377
  
677-697
  
tcctacttggttcggtacatggg
  
Human HeLa
  
View, Order
  

p66 ShcA (3)   
HSU73377
  
236-256
  
CGGAATGAGTCTCTGTCATCGTC
  
Human HeLa
  
View, Order
  

Sphingosine kinase 1 (hSK1) (15)
  

  
70 nt downstream of start codon
  
AAGAGCUGCAAGGCCUUGCCC
  
HUVEC
  
View, Order
  
View
Steroid receptor coactivator (SRC-1) (30)         CCUCAGGGCAGAGAACCAUCUTT   
Ishikawa
     
ß-tubulin (6)   
AF312873
  
3'-UTR
  
GACAGAGCCAAGUGGACUCAC
  
Mouse P19
  

  

Tissue Factor (hTF) (18)   
M16553
  

  
AAGCGCUUCAGGCACUACAAA
  
HeLa, 293, Cos-1, HaCaT
  
View, Order
  
View
Tissue Factor (hTF) (18)   
M16553
  

  
AAGAAGCAGACGUACUUGGCA
  
HeLa, 293, Cos-1, HaCaT
  
View, Order
  
View
ß-transducin repeat-containing protein (ß-TrCP), human (10)   

  

  
GUGGAAUUUGUGGAACAUCTT
  
293
  
View, Order
  
View
TSG101 (a ubiquitin enzyme 2 variant protein) (14)   

  
413-433
  
AACCUCCAGUCUUCUCUCGUC
  
Human HeLa-M & HeLa KK3
  
View, Order
  
View

VASP (1)
  

XM_009141
  

1087-1109
  

aaagaggaaatcattgaagcc
  
Human HeLa
  
View, Order
  
View

vimentin (1)
  

NM_003380
  

346-368
  

aactacatcgacaaggtgcgc
(No silencing observed; 1,2)
  
Human HeLa
  
View, Order
  
View

vimentin (1)
  

NM_003380
  

1145-1167
  

aataccaagacctgctcaatg
  
Human HeLa
  
View, Order
  
View

vimentin (1)
  

NM_003380
  

863-885
  

aagaatggtacaaatccaagt
  
Human HeLa
  
View, Order
  
View

vimentin (1)
  

NM_003380
  

1037-1059
  

aaatggaagagaactttgccg
  
Human HeLa
  
View, Order
  
View

vinculin, mouse (1)
  

L18880
  

2923-2945
  

aagcggattagaaccaatctc
  
Mouse 3T3
  
View, Order
  
View

zyxin, mouse (1)
  

X99063
  

1357-1379
  

aaagcctaccacccacagtgc
  
Mouse 3T3
  
View, Order
  
View
~undefined"View" shows the sense and antisense strands of the siRNA corresponding to the Gene Target. "Order" leads to a custom siRNA order form with the appropriate siRNA sequence pre-filled out. See Ambion's Custom siRNA Oligonucleotide Synthesis Service for details.

~undefinedTemplate_sequences_for_the_Silencer™ siRNA Construction Kit.

References

1. Harborth J, Elbashir SM, Bechert K, Tuschl T, and Weber K. (2001) Identification of essential genes in cultured mammalian cells using small interfering RNAs. J Cell Science 114: 4557-4565.

2. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, and Tuschl T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494-498.

3. Kisielow M, Kleiner S, Nagasawa M, Faisal A, and Nagamine Y. (2002) Isoform-specific knockdown and expression of adapter protein ShcA using small interfering RNA. Biochem. J. 363:1-5.

4. Cortez D, Guntuku S, Qin J, and Elledge SJ. (2001) ATR and ATRIP: partners in checkpoint signaling. Science 294:1713-1716.

5. Zhou Y, Ching Y-P, Kok KH, Kung H-F, Jin D-Y. (2002). Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA. Nucleic Acids Res 30(7):1664-1669.

6. Yu J-Y, DeRuiter SL, Turner DL. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99(9):6047-6052.

7. Zou L, Cortez D, and Elledge SJ. (2002). Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin. Genes & Devel. 16:198-208.

8. Stucke VM, Sillje HHW, Arnaud L, and Nigg EA. (2002). Human Mps1 kinase is required for the spindle assembly checkpoint but not for centrosome duplication. EMBO 21(7):1723-1732.

9. Donze O, and Picard D. (2002). RNA interference in mammalian cells using siRNAs synthesized with T7 polymerase. Nucl. Acids Res. 30(10): e46.

10. Fong A, and Sun S-C. (May 6, 2002). Genetic evidence for the essential role of beta-transducin repeat-containing protein in the inducible processing of NF-kB2/p100. J. Biol. Chem. (preprint).

11. Williams R, and Rubin GM. (2002). ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos. Proc. Natl. Acad. Sci. USA 99(10):6889-6894.

12. Wu F, Yan W, Pan J, Morser J, and Wu Q. (2002). Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes. J. Biol. Chem. 277(19):16900-16905.

13. Bai, X, Zhou, D, Brown, JR, Crawford, BE, Hennet, T, and Esko, JD. (2001). Biosynthesis of the linkage region of glycosaminoglycans. J. Biol. Chem. 276(51):48189-48195.

14. Hewitt EW, Duncan L, Mufti D, Baker J, Stevenson PG, and Lehner PJ. (2002). Ubiquitylation of MHC class I by the K3 viral protein signals internalization and TSG101-dependent degradation. EMBO 21(10): 2418-2429.

15. Ancellin, N, Colmont, C, Su, J, Li, Q, Mittereder, N, Chae, S-S, Stefansson, S, Liau, G, and Hla, T. (2002). Extracelllular export of sphingosine kinase-1 enzyme. J. Biol. Chem. 277(8):6667-6675.

16. Li L, Mao J, Sun L, Liu W, and Wu D. (2002). Second cysteine-rich domain of Dickkopf-2 activates canonical Wnt signaling pathway via LRP-6 independently of Dishevelled. J. Biol. Chem. 277(8):5977-5981.

17. Eide EJ, Bielhaber EL, Hinz W, and Virshup DM. (2002). The circadian regulatory proteins BMAL1 and Cryptochromes are substrates of casein kinase I epsilon. J. Biol. Chem. 277(19):17248-17254.

18. Holen T, Amarzguioui M, Wiige, MT, Babaie E, and Prydz H. (2002). Positional effects of short interfering RNAs targeting the human coagulation trigger tissue factor. Nucl. Acids Res. 30(8):1757-1766.

19. Bakker J, Lin X, and Nelson WG. (2002). Methyl-CpG binding domain protein 2 represses transcription from hypermethylated p-class glutathione-S-transferase gene promoters in hepatocellular carcinoma cells. J. Biol. Chem. (in press).

20. Matuliene J, and Kuriyama R. (17 April 2002). Kinesin-like protein CHO1 is required for the formation of midbody matrix and the completion of cytokinesis in mammalian cells. Mol. Biol. Cell. (in press).

21. Yu, J-Y, DeRuiter, SL, Turner, DL. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99(9):6047-6052.

22. Shang Y and Brown M. (2002). Molecular determinants for the tissue specificity of SERMs. Science 295:2465-2468.

23. Mailand N, Lukas C, Kaiser BK, Jackson PK, Bartek J, and Lukas J. (2002). Deregulated human Cdc14A phosphatase disrupts centrosome separation and chromosome segregation. Nature Cell Biol. 4:317-322.

24. Chang T-S, Jeong W, Choi SY, Yu S, Kang SW, and Rhee SG. (2002). Regulation of peroxiredoxin I activity by Cdc2-mediated phosphorylation. J. Biol. Chem. 277(28):25370-25376.

25. Liu J, Yao F, Wu R, Morgan M, Thorburn A, Finely RL, Chen YQ. (2002). Mediation of the DCC apoptotic signal by DIP13 alpha. J. Biol. Chem. 277(29):26281-26285.

26. Koepp DM, Schaefer LK, Ye X, Keyomarsi K, Chu C, Harper JW, and Elledge SJ. (2001). Phosphorylation-dependent ubiquitination of cyclin E by the SCF-Fbw7 ubiquitin ligase. Science 294(5540):173-177.

27. Bai, X, Zhou, D, Brown, JR, Crawford, BE, Hennet, T, and Esko, JD. (2001). Biosynthesis of the linkage region of glycosaminoglycans. J. Biol. Chem. 276(51):48189-48195.

28. Martins et al. (2002). The serine protease Omi/HtrA2 regulates apoptosis by binding XIAP through a reaper like motif. J. Biol. Chem. 277:439-444.

29. Hirai H and Wang H-G. (2002). A role of the C-terminal region of human Rad9 (hRad9) in nuclear transport of the hRad9 checkpoint complex. J. Biol. Chem. 277(28):25722-25727.

30. Shang Y and Brown M. (2002). Molecular determinants for the tissue specificity of SERMs. Science 295:2465-2468.

31. Lassus P, Opitz-Araya X, and Lazebnik Y. (2002). Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 297:1352-1354.

32. Scherr M, Battmer K, Winkler T, Heidenreich O, Ganser A, and Eder M. (2002). Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood (online September 26, 2002 issue)

33. Heinonen JE, Smith CIE, and Nore BF. (2002). Silencing of Bruton's tyrosine kinase (Btk) using short interfering RNA duplexes (siRNA). FEBS Lett 527:274-278.

34. Lassus P, Opitz-Araya X, and Lazebnik Y. (2002). Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization. Science 297:1352-1354.

35. Zhang D, Li F, Weidner D, Mnjoyan ZH, and Fujise K. (2002). Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and fortilin. J. Biol. Chem. 277(40):37430-37438.

36. Martin-Lluesma S, Stucke VM, and Nigg EA. (2002). Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 297:2267-2270.

37. Liu X and Erikson RL. (2002). Activation of Cdc2/cyclin B and inhibition of centromere amplification in cells depleted of Plk1 by siRNA. Proc. Natl. Acad. Sci. USA 99(13):8672-8676.

38. Jiang M and Milner J. (2002). Selective silencing of viral gene expression in HPV-positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene 21:6041-6048.

39. Boehm M, Yoshimoto T, Crook MF, Nallamshetty S, True A, Nabel G, and Nabel EG. (2002). A growth factor-dependent nuclear kinase phosphorylates p27kip1 and regulates cell cycle progression. EMBO J. 21(13):3390-3401.

40. Tsuneoka M, Koda Y, Soejima M, Teye K, and Kimura H. (2002). A novel Myc target gene, mina53, that is involved in cell proliferation. J. Biol. Chem. 277(38):35450-35459.

41. Martin-Lluesma S, Stucke VM, and Nigg EA. (2002). Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 297:2267-2270.

42. Krichevsky AM and Kosik KS. (2002). RNAi functions in cultured mammalian neurons. Proc. Natl. Acad. Sci USA 99(18):11926-11929.

43. Zhang D, Li F, Weidner D, Mnjoyan ZH, and Fujise K. (2002). Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and fortilin. J. Biol. Chem. 277(40):37430-37438.

44. Surka MC, Tsang CW, and Trimble WS. (2002). The mammalian septin MSF localizes with microtubules and is required for completion of cytokinesis. Mol Biol. Cell 13:3532-3545.

45. Edbauer D, Winkler E, Haass C, and Steiner H. (2002). Presenilin and nicastrin regulate each other and determine amyloid b-peptide production via complex formation. Proc. Natl. Acad. Sci. USA 99(13):8666-8671.

46. Prasanth SG, Prasanth KV, and Stillman B. (2002). Orc6 involved in DNA replication, chromosome segregation, and cytokinesis. Science 297:1026-1031.

47. Chevrier V, Piel M, Collomb N, Saoudi Y, Frank R, Paintrand M, Narumiya S, Bornens M, and Job D. (2002). The Rho-associated protein kinase p160ROCK is required for centrosome positioning. J. Cell Biol. 157:807-817.